Advances and challenges in logical modeling of cell cycle regulation: perspective for multi-scale, integrative yeast cell models
This article discusses the development of logical modeling of cell cycle regulation in Saccharomyces cerevisiae and perspectives for its integration with cellular networks for multi-scale models.
DNA motor protein-mediated replication fork regression can restart stalled replication but may also generate dangerous outcomes. Positive and negative regulators can influence the outcomes depending on the specific molecular settings.
Genome-wide high-throughput screens are powerful tools that enable identification of established and novel genes involved in recombinant protein expression.
Fermentation assays reveal differences in sugar and (off-) flavor metabolism across different Brettanomyces bruxellensis strains
Sugar consumption and (off-)flavor production by Brettanomyces bruxellensis is dependent on both the yeast strain and medium composition.
Sch9p and Gcn4p profoundly impacted wine yeast physiology during grape juice fermentation, and not only affected nitrogen but also carbon metabolism, which affected growth, cell longevity and glycerol production.
Improving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double-bond reductase
Replacement of essential yeast reductase with corresponding gene from apple allows more efficient production of flavonoids by eliminating formation of side products.
Functional analysis of Debaryomyces hansenii Rpn4 on a genetic background of Saccharomyces cerevisiae
Debaryomyces hansenii DEHA2D12848 gene encodes a transcriptional activator for proteasomal genes with unique transactivation domain.
Phylogenetic data on Saccharomyces uvarum strains, isolated for the first time from Patagonian apple chicha suggest that these strains might have been introduced in Patagonia during the domestication of apples by Mapuche communities.
Elimination of sucrose transport and hydrolysis in Saccharomyces cerevisiae: a platform strain for engineering sucrose metabolism
A yeast strain without functional sucrose transport and hydrolysis was constructed and is now ready to be used for metabolic engineering strategies and for fundamental studies into sucrose metabolism.