Drosophila models of phosphatidylinositol glycan biosynthesis class A congenital disorder of glycosylation (PIGA-CDG) mirror patient phenotypes

Abstract Mutations in the phosphatidylinositol glycan biosynthesis class A (PIGA) gene cause a rare, X-linked recessive congenital disorder of glycosylation. Phosphatidylinositol glycan biosynthesis class A congenital disorder of glycosylation (PIGA-CDG) is characterized by seizures, intellectual and developmental delay, and congenital malformations. The PIGA gene encodes an enzyme involved in the first step of glycosylphosphatidylinositol (GPI) anchor biosynthesis. There are over 100 GPI-anchored proteins that attach to the cell surface and are involved in cell signaling, immunity, and adhesion. Little is known about the pathophysiology of PIGA-CDG. Here, we describe the first Drosophila model of PIGA-CDG and demonstrate that loss of PIG-A function in Drosophila accurately models the human disease. As expected, complete loss of PIG-A function is larval lethal. Heterozygous null animals appear healthy but, when challenged, have a seizure phenotype similar to what is observed in patients. To identify the cell-type specific contributions to disease, we generated neuron- and glia-specific knockdown of PIG-A. Neuron-specific knockdown resulted in reduced lifespan and a number of neurological phenotypes but no seizure phenotype. Glia-knockdown also reduced lifespan and, notably, resulted in a very strong seizure phenotype. RNA sequencing analyses demonstrated that there are fundamentally different molecular processes that are disrupted when PIG-A function is eliminated in different cell types. In particular, loss of PIG-A in neurons resulted in upregulation of glycolysis, but loss of PIG-A in glia resulted in upregulation of protein translation machinery. Here, we demonstrate that Drosophila is a good model of PIGA-CDG and provide new data resources for future study of PIGA-CDG and other GPI anchor disorders.


Introduction
Phosphatidylinositol glycan biosynthesis class A congenital disorder of glycosylation (PIGA-CDG) is an ultrarare, X-linked recessive disorder caused by partial loss of function mutations in the PIGA gene (Johnston et al. 2012;Kato et al. 2014;van der Crabben et al. 2014;Bayat et al. 2020).Patients with PIGA-CDG display a range of symptoms affecting many systems including neurological abnormalities, muscular abnormalities, and skeletal abnormalities, with most patients presenting with seizures, hypotonia, and neurodevelopmental delay (Bayat et al. 2020).There are fewer than 100 reported patients with PIGA-CDG with ∼40 different mutations (Bayat et al. 2020).The underlying mechanisms of PIGA-CDG are not understood, and current treatment options are limited.Most treatments for CDGs focus on alleviating symptoms, rather than correcting the cause of the disorder.
Glycosylphosphatidylinositol (GPI) anchor synthesis is a highly conserved pathway involving over 30 proteins which builds a sugar chain on a phosphatidylinositol molecule in a stepwise manner.The PIGA gene encodes phosphatidylinositol glycan biosynthesis class A, the catalytic enzyme involved in the first step GPI anchor synthesis (Miyata et al. 1993;Watanabe et al. 1998;Kinoshita and Inoue 2000).The first steps of GPI anchor biosynthesis occur on the cytoplasmic side of the endoplasmic reticulum (ER).In the first step, PIGA transfers an N-acetylglucosamine (GlcNAc) from uridine 5′-diphospho N-acetylglucosamine (UDP-GlcNAc) to an existing phosphatidylinositol (PI) within the ER membrane, generating the first intermediate of GPI anchor synthesis, N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) (Orlean and Menon 2007;Kinoshita et al. 2008).
GPI anchors attach over 100 proteins to the surface of the cell that are involved in many functions including cell signaling, immunity, and adhesion.Loss of PIGA function leads to decreased surface expression of GPI-anchored proteins (Lukacs et al. 2020;Liu et al. 2021).Typical PIGA-CDG patients display 5-15% of normal GPI-anchored proteins on the cell surface (Bayat et al. 2020).GPI-anchored protein precursors have a C-terminal signal sequence that is normally cleaved when the protein is attached to the anchor (Kinoshita 2020).If GPI anchor synthesis is inhibited before addition of the first mannose to the anchor (such as in PIGA-CDG), the C-terminal sequence is not cleaved from the protein, and the protein is recognized as a misfolded protein and sent to be degraded by the proteasome (Kinoshita 2020).It is unclear if the symptoms observed in PIGA-CDG are due to cellular stress caused by the misfolded proteins or if they are due to loss of specific GPI-anchored proteins, although it is likely due to a combination of both factors.
There are only a handful of PIGA cellular or animal model studies.Cultured mouse and human cells with PIGA null mutations are viable but show decreased abundance of GPI-anchored proteins on the surface of the cell (Lukacs et al. 2020;Liu et al. 2021).While cell culture systems are useful for investigating the cellular effects of loss of PIGA, many effects are missed in cell models because there are many cell-type specific GPI-anchored proteins.Mouse models have also been used to investigate PIGA-CDG (Kawagoe et al. 1996;Lukacs et al. 2019Lukacs et al. , 2020;;Kandasamy et al. 2021;Jangid et al. 2022).Global knockout of Piga in mice is embryonic lethal (Kawagoe et al. 1996).Conditional knockout of Piga in the mouse central nervous system results in ataxia and degenerative tremors (Lukacs et al. 2020).To date, no Drosophila models have been reported.
Here, we generated Drosophila models to investigate the pathophysiology of phenotypes observed in PIGA-CDG.PIG-A is the single, well-conserved Drosophila ortholog of human PIGA (FlyBase; DIOPT v9.1 Score: 14/14) (Gramates et al. 2022).Homozygous PIG-A null alleles result in larval lethality, but the heterozygotes are viable and have a seizure phenotype similar to what is observed in patients.Cell-specific knockdowns (KD) in glia and neurons lead to distinct neurological phenotypes.KD in the neurons causes neuromuscular defects while KD in glia results in seizures.Transcriptome analyses on these different models showed distinct transcriptomic signatures when PIGA function is reduced in different cell types.Together, this is the first report of a Drosophila model of PIGA-CDG and will be a rich resource for future studies on the pathogenesis of this understudied rare disorder.

Drosophila melanogaster fly stocks
All stocks were maintained under standard laboratory conditions on agar-dextrose-yeast medium at 25°C on a 12 h light/dark cycle.Male flies were used in all experiments because initial pilot experiments with both sexes did not detect any significant sex differences.The following strains are from Bloomington Drosophila Stock Center: PIG-A RNAi (62696), tub-GAL4 (5138), repo-GAL4 (7415), and elav-GAL4 (46655).The corresponding attP40 control strain was used as our wild-type control.The PIG-A null allele (PIG-A −/− and PIG-A +/− ) was generated as a PIG-A Kozak-miniGAL4 allele (Kanca et al. 2019) using the sgRNAs TATGTGG TATGTCAAATTACTGG and TTGGCTAGTTGATGGAAGAATGG.

Quantitative PCR
Total RNA was extracted from tubulin-GAL4 > PIG-A RNAi (Bloomington Drosophila Stock Center: 62696) and control 3rd instar larvae using TRIzol Reagent (ThermoFisher Cat #15596026) followed by Direct-zol RNA MiniPrep with DNAse step (Zymo Research R2051).Each biological replicate contained 15-20 larvae.One microgram of RNA was used to synthesize cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB Cat #E6560L).Fifty nanogram of cDNA was used to perform quantitative PCR (qPCR) with PowerUp SYBR Green Master Mix (ThermoFisher Cat #A25741).All protocols were followed according to the manufacturer's instructions.qPCR was run on a QuantStudio 3. Fold change of gene expression was calculated using the Delta-Delta Ct method.Primers used are from FlyPrimerBank (Primer ID #PP29152).

Larval tracking
Larval size and survival was tracked using timed egg lays.Heterozygous PIG-A +/− flies were mated and allowed to lay for 8 h.Hours post egg lay were calculated from the time mated females were removed, at the end of the 8 h egg lay period.Homozygous null PIG-A −/− larvae can be distinguished from heterozygous PIG-A +/− larvae by the presence of a balancer chromosome (BDSC 9325) that carries an actin-GFP marker in the heterozygous larvae.Larvae were genotyped and sorted by the presence of the GFP marker.Larvae were imaged at 2.5× magnification using a Leica EC3 camera.Larval size was quantified using ImageJ as previously described (Hope et al. 2022).All larval measurements were performed with at least 20 larvae per genotype.

Lifespan
Lifespan was monitored daily.Ten flies were placed in each vial.All flies were kept at 25°C and maintained on a 12:12 light dark cycle (lights on at 7 AM).Flies were monitored for death once a day until all flies were dead.Survival analysis was performed using default settings in the Survival package in R (R version 4.2.0;survival package version 3.3-1; running under Windows 10 x64).All lifespan experiments were performed with at least 125 flies per genotype.

Climbing
Flies were maintained under standard conditions for at least 1 day after CO 2 collection.All flies that were tested were 3-5 days of age.Ten flies were placed in empty vials and allowed to rest for 10 min.Vials were tapped to drop all flies to the bottom and flies were given 20 s to climb.At the end of 20 s, flies were counted in the bottom, middle, and top third of the vials.Proportion was calculated based on number of flies in each section of the vial and total flies in vial.All climbing experiments were performed with at least 50 flies per genotype.

Bang sensitivity
Flies were maintained under standard conditions for at least 1 day after CO 2 collection.All flies that were tested were 3-5 days of age.Ten flies were placed in empty vials and allowed to rest for 10 min.Vials were vortexed at full speed for 10 s and seizure recovery was observed.Flies were considered to be seizing until they were walking around.All bang sensitivity experiments were performed with at least 100 flies per genotype.

RNA sequencing
mRNA sequencing was performed on total RNA from 10 heads of male control or knockdown 3-day-old flies.Neuron-and glialspecific knockdown had their own controls.All groups were sequenced in triplicate, for a total of 12 samples.
RNA was extracted using a Direct-zol RNA MiniPrep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat #15596026) and including the DNAse step.Samples were prepared and sequenced by the Huntsman Cancer Institute High-Throughput Genomics Core.Samples were sequenced on the NovaSeq 50 × 50 bp Sequencing, for ∼25 million paired reads per sample.Fastq files were trimmed using seqtk v1.2 software (for Fastq and processed files, see GEO repository: GSE241512).
RNA sequencing (RNAseq) reads were aligned to the Drosophila melanogaster reference genome (assembly BDGP Release 6) using Bowtie2 v2.2.9 software (Langmead and Salzberg 2012), and alignment files were sorted and converted using SAMtools v1.12 (Li et al. 2009).Read counts were normalized using the default normalization method in DESeq2 (Love et al. 2014) package in R. Differential gene expression was assessed using linear models in the DESeq2 package.Genes were considered significantly differentially expressed if the adjusted P-value ≤ 0.05 and the fold change magnitude was ≥1.5 (Log2 fold change ≥0.585 or ≤−0.585).Gene Ontology (GO) analyses (Ashburner et al. 2000;Gene Ontology et al. 2023) were performed using standard tools at www.geneontology.org.

Complete loss of PIG-A is lethal
We obtained a null allele of PIG-A, wherein the coding sequence is replaced by a GAL4 cassette (Kanca et al. 2019).Homozygous PIG-A −/− adult flies are never observed.To determine when the PIG-A −/− larvae die, we tracked their growth during larval development (Fig. 1a).PIG-A −/− are indistinguishable from heterozygous controls in the 3rd instar larval stage, up to 96 h after egg laying.Beginning around this time, PIG-A −/− larval growth slows and is significantly smaller than heterozygous controls.PIG-A −/− larvae never progress to pupation and languish as 3rd instar, while heterozygous control larvae pupate and eclose as adults.
We also evaluated ubiquitous knockdown of PIG-A using a tubulin-GAL4 driver to express a UAS-RNAi.We also never observed adult knockdown flies.Unlike the null flies, the ubiquitous KD pupae are indistinguishable from wild-type controls and do not die during development.Despite this, the ubiquitous KD animals still all die during pupation.The slight difference in survival and development suggests that the RNAi knockdown is not a complete knockdown, and some residual expression remains.In line with this, qRT-PCR of PIG-A transcript in ubiquitous KD larvae indicate that there is significant reduction in expression, with ∼39% residual transcript (39.6 ± 5.9; P < 0.0001) (Supplementary Fig. 1).

Haploinsufficiency of PIG-A results in seizures
PIG-A +/− flies develop normally and eclose as adults at the expected Mendelian ratio.However, we reasoned that these heterozygous null flies are more similar to PIGA-CDG patients than the homozygous nulls.Because PIGA is X-linked in humans, all reported PIGA-CDG patients carry a single partial loss-of-function mutation, resulting in 50% or less activity (Johnston et al. 2012;Bayat et al. 2020).To evaluate if the PIG-A +/− flies could model patient phenotypes and have neurological deficits, we first tested them for climbing ability, a general measure of nervous system function.The standard protocol for evaluating climbing ability entails tapping the flies to the bottom of the vial and measuring how long it takes to climb to the top.Upon tapping down these flies, we noticed that a subset would display seizure-like behavior, preventing any meaningful climbing assessment.To formally test if PIG-A +/− flies have seizures, we performed the bang-sensitive assay, a commonly used measure of seizure susceptibility in flies.We found that on average, 35% of PIG-A +/− flies have seizure activity following bang-sensitive testing, compared with zero seizures in the WT flies (0.35 ± 0.04; P = 1.4 × 10 −9 ) (Fig. 1b, Supplementary Videos 1 and 2).This suggests that the heterozygous null flies may model some of the prominent patient phenotypes.

Cell-type specific knockdown of PIG-A
Because most of the prominent phenotypes associated with PIGA-CDG are neurological (Bayat et al. 2020) and to begin to uncover the cellular origins of the phenotypes, we performed neuronor glial-specific knockdown of PIG-A to model neurological phenotypes observed in patients.To generate neuron-specific knockdown of PIGA, we used the pan-neuronal driver, elav-GAL4, to drive PIG-A RNAi (same as described above) in all neurons.Neuron-specific knockdown flies eclosed at expected Mendelian ratio but have a shorter lifespan than control flies (P < 2.0 × 10 −16 ) (Fig. 2a).Fifty percent of neuron-specific knockdown flies are dead by 40 days post eclosion, while more than 90% of control flies are still living.Neuron-specific knockdown flies display a climbing defect where nearly 100% of flies fail to climb to the top of vial in 20 s (Fig. 2b).Control flies easily climb to the top in the same time interval (P < 1.3 × 10 −3 ).Neuron-specific knockdown flies also display a degenerative erect wing phenotype (Fig. 2c).They eclose with normal wing posture, but over 5 days, all the flies develop an erect wing phenotype.Control flies do not display a wing phenotype over this same period (P < 3.0 × 10 −3 ).Strikingly, neuron-specific knockdown flies do not show a seizure phenotype upon treatment with a bang-sensitive protocol.
To generate glial-specific knockdown of PIG-A, we used the panglial driver, repo-GAL4, to drive PIG-A RNAi in all glia.Glia-specific knockdown flies also eclosed at an expected Mendelian ratio but have a shorter life span than control flies.Fifty percent of glia-specific knockdown flies are dead at 60 days post eclosion, while nearly 80% of control flies are living (P < 2.0 × 10 −16 ) (Fig. 3a).Glia-specific knockdown flies have a slower rate of death than neuron-specific knockdown flies.Glia-specific knockdown flies have a severe seizure phenotype upon bang sensitivity testing (Fig. 3b, Supplementary Videos 3 and 4).Seventy percent of glia-specific knockdown flies display a very severe seizure phenotype where the flies completely pass out for nearly a minute before recovery.Fewer than 1% of control flies show a seizure phenotype with the same testing.This is likely an underestimate of the proportion of flies that seize because it appears that seizures can easily be elicited in glia-specific knockdown flies.We often observe flies that have passed out in a vial that has not been subject to bang-sensitive testing.We also observe flies that pass out when someone walks by the vials.Thus, the 70% seizure rate is an underestimation because at any time, some of the flies are likely in a refractory period after a nonelicited seizure.Because of this severe seizure phenotype, we were unable to assay movement-related deficits.Finally, ∼60% of glia-specific knockdown flies have a wrinkled, partially inflated wing phenotype, compared with normal wings in 100% of control flies (Fig. 3c and d).The remaining ∼40% of glia-specific knockdown flies have wild-type normal-appearing wings.

Transcriptomic data
To determine how loss of PIG-A in neurons vs glia affects gene expression in the brain, we performed RNAseq analyses on whole heads.Neuron-specific knockdown heads showed a modest change in expression with 191 genes upregulated and 104 genes downregulated compared with control heads (Fig. 4a; Supplementary Table 1).Glia-specific knockdown heads showed a more substantial change in expression with 493 genes upregulated and 257 genes downregulated compared with control heads (Fig. 4b; Supplementary Table 1).
We next examined the overlapping transcriptomic profile between neuron-and glia-specific knockdown (Fig. 4c; Supplementary Table 3).Forty-one genes are commonly upregulated at least 1.5-fold, between the 2 data sets.GO analyses of these common upregulated genes identified alpha-amino acid catabolic process (GO: 1901606; fold enrichment: 36.2;P < 3.58 × 10 −4 ) and cellular amino acid catabolic process (GO: 0009063; fold enrichment: 33.1; P < 4.84 × 10 −2 ).Each independent set of upregulated genes also showed upregulation for amino acid metabolism-related functions.There is strong correlation in the degree of upregulation between these overlapping genes (r 2 = 0.90; P = 1.3 × 10 −15 ).We expanded the analyses to genes that were upregulated in either data set with a more relaxed 1.25-fold upregulation in the other data set to look more broadly at overlapping upregulated genes.While amino acid metabolism-related functions remain, we find enrichment for SRP-dependent cotranslational protein targeting to membrane, translocation (GO: 0006616; fold enrichment: 35.5;P < 5.31 × 10 −2 ) and hexose metabolic process (GO: 0019318; fold enrichment: 10.1; P < 2.0 × 10 −2 ).Each of these was identified in glia and neurons, respectively, but not the other.The degree of upregulation between these genes is still positive between neurons and glia (r 2 = 0.56; P = 5.3 × 10 −16 ).

Discussion
Here, we report the first Drosophila model of a disorder of GPI anchor biosynthesis.PIGA is the first step in GPI anchor synthesis and causes neurodevelopmental delay, movement disorder, and epilepsy (Bayat et al. 2020).Using various genetic techniques, we show that we can model each of these phenotypes in Drosophila.The Drosophila models mirror what has been reported in mouse models of PIGA-CDG, but importantly, the Drosophila models also display certain patient phenotypes that have not been reported in mice.
As expected, complete loss of PIG-A function is larval lethal in Drosophila.It is unlikely that proper GPI anchor biosynthesis can occur without PIGA.In line with this, complete Piga knockout in mice are early embryonic lethal (Kawagoe et al. 1996).Further, no PIGA-CDG patients have been reported with predicted null mutations (Bayat et al. 2020).While a few protein truncating mutations have been reported, they all produce truncated mutant protein.It was surprising that the Drosophila null mutants survived through 3rd instar larval development.We suspect that this was likely possible because of maternal deposition of PIGA protein itself and a variety of GPI-anchored proteins required for proper development.More work is needed to determine if this is the case.
Heterozygous null Drosophila have 50% function, which is similar to the level of residual activity predicted in PIGA-CDG Because PIGA is X-linked in humans, only males are affected, and they carry partial loss-of-function alleles that are predicted to  have <50% function (Bayat et al. 2020).Strikingly, these heterozygous null PIG-A Drosophila show a strong seizure phenotype that is very easily elicited.Due to the severe seizure phenotype, we were not able to test if movement or other neurological phenotypes were normal.However, we did note that when flies are not seizing, they are climbing normally like wild-type flies.The ease at which these heterozygous flies have seizures matches a recent description of mosaic Piga heterozygous knockout female mice (X-linked, but 1 copy is randomly inactivated) that appear to also have easily elicited seizures when they fall (Lukacs et al. 2020).These heterozygous null flies will be a useful tool for studying the pathogenesis of seizures in PIGA-CDG and for testing possible small molecule therapeutics.Because of the lethality and the inability to probe for other phenotypes in the homozygous null flies, we opted to use RNAi against PIG-A for further analyses.While knockdown by this RNAi was >60%, it is difficult to compare this with the heterozygous null, as qPCR was performed on whole larvae, a mixture of different cells that likely have different knockdown efficiencies.However, the fact that ubiquitous knockdown resulted in similar lethality as the null suggests that at least for the critical cells, RNAi is quite effective.Neuron-and glia-specific RNAi allowed us to model movement disorder and neuromuscular defects and seizures, respectively, similar to what is observed in PIGA-CDG (Bayat et al. 2020).Surprisingly, neuron-specific knockdown did not produce observable seizures.We tried a number of different ways to elicit seizures beyond the bang sensitivity test and were not able to observe any seizure activity.Glial-specific knockdown generated flies with a profound seizure phenotype, much more severe than the heterozygous null flies.Glial-specific knockdown resulted in flies that likely have spontaneous seizures.The majority of flies seize upon bang sensitivity testing.However, we noticed that many of the flies will drop and seize even if a lab member walks by the vial.This separation of patient-relevant phenotypes in different cell types suggests that PIGA-CDG has a complex etiology and that different cell types have different requirements for GPI anchor biosynthesis.
Despite these striking differences between neurons and glia, it's important to note that it is difficult to make a direct comparison between the 2 models.For example, it is not possible to easily assess whether neurons and glia have similar knockdown levels.It is possible the some of the differences between the models could arise from different levels of knockdown.It is also likely that loss of PIG-A in neurons vs glia can have profoundly different effects on cells that are normal.Nevertheless, these models will be useful in determining the important of PIGA function and modeling human disease.
To begin to understand some of the molecular consequences of losing PIG-A function, we performed RNAseq on heads from neuron-and glia-specific knockdown flies.While this was an imperfect experiment because the transcriptome signatures are from a mix of normal and knockdown cells, we still observed very strong, specific changes that are informative.It should be noted that because we used RNAi, the transcriptome responses reflect partial loss of PIGA function, rather than complete loss of function.Overall, glia-specific knockdown resulted in more differentially expressed genes, as compared with the neuron-specific knockdown.Perhaps, this makes sense, as the glial-specific knockdown flies have more severe phenotypes (though different) than the neuron-specific knockdown flies.The transcriptomes from these 2 models produced very specific but unique patterns.When we knockdown PIG-A in neurons, we find a near uniform upregulation of enzymes in the entire glycolysis pathway.We also found upregulation of enzymes responsible for amino acid metabolism.It is likely that this is not an increase in energy production, as enzymes in the TCA cycle remain mostly unchanged.Rather, we think that the cells are responding to a loss of GPI anchor production.Many products of glycolysis and amino acid metabolism are the substrates for the PIGA-catalyzed synthesis of the UDP-GlcNAc precursor and GlcNAc-PI (Chiaradonna et al. 2018).In fact, levels of UDP-GlcNAc act as a "sensor" to regulate glycolysis and other metabolic pathways (Chiaradonna et al. 2018).This suggests that when neurons have reduced PIGA function, cells in the brain are upregulating pathways that may provide the precursor molecules needed to ramp up GPI anchor biosynthesis.
Glial-specific knockdown flies are likely ramping up the production of protein translational and folding machinery in the brain.It appears that the cells are responding to a reduction in GPI-anchored protein production and increasing machinery to produce more GPI-anchored proteins.Nearly all the components of ER-associated translation are upregulated, including the SRP complex and the entire SEC21 translocon complex.Further, many genes encoding ER-resident proteins involved in folding processing newly translated proteins are upregulated.This does not appear to be a response to ER stress, as other classic ER stress response genes, including those involved in ERAD, are unchanged.When glia have reduced PIGA function, cells in the brain respond by increasing ER-associated translation.
There were very few genes that showed the same direction of change between the 2 data sets.But the genes in common were very strongly correlated, suggesting a core response.The top 10 genes in this overlapping set all show at least 4-fold change in expression.However, these strong response genes mostly have unknown function with no human orthologs.We observed that the commonly upregulated genes show enrichment for amino acid metabolism.This enrichment is also observed in the neuronspecific knockdown but not the glia.Upon manual curation, many of the genes that are commonly upregulated are enzymes related to energy metabolism, which again makes sense for the neuron-specific knockdown.However, it suggests that there may also be changes related to metabolism and glycolysis in the glia-specific knockdowns as well but does not reach the enrichment observed in the neuron-specific knockdown.It needs to be noted that all the major glycolysis enzymes are unchanged in the glia-specific knockdown.More work is needed to understand the cellular origin of the changes uncovered by RNAseq.Future analyses involving metabolomics and proteomics will help to elucidate the changes that occur when the GPI anchor synthesis is disrupted.
In this study, we report the initial analyses of the first PIGA-CDG models in Drosophila.This study reinforces that Drosophila can serve as an efficient tool to accurately model CDGs and other rare neurodevelopmental disorders.The data here set the stage for future modifier and drug screen studies to help identify potential therapeutic strategies for PIGA-CDG.

Fig. 1 .
Fig. 1.PIG-A null phenotypes.a) PIG-A −/− larvae are smaller than PIG-A +/− control larvae and they fail to eclose.b) ∼35% of PIG-A +/− flies display seizures upon bang-sensitive testing.Each point represents a single experiment with at least 30 flies.Wild-type flies do not have seizures.

Fig. 6 .
Fig. 6.The glycolysis pathway is upregulated in neuron-specific knockdown of PIG-A.Nearly all the components of the glycolysis pathway are upregulated in neuron-specific knockdown of PIG-A.Numbers indicate fold change.nc, not changed.