In the originally published version of this article there was an error in Figure 2. The image used for the western blot of the immunoprecipitations shown in panel C was incorrect, as an alternative exposure of the blot shown in panel B was mistakenly used. The version of Figure 2 shown here has been amended to show the correct image of the western blot in panel C.
ERdj5 interacts with WTrod opsin and ERdj5 reductase and J domain mutants affect its traffic. (A) SK-N-SH cells were transfected with Rho-GFP (green) alone (no ERdj5-HA) or co-transfected at 1 : 1 ratio with ERdj5-HA, ERdj5-SS-HA or ERdj5-H63Q-HA, as indicated. Cells were fixed and immunostained with an antibody against HA for detection of ERdj5 localization (redmiddle panel). Arrows highlight ER retention of Rho-GFP. Scale bar: 10 mm. (B-C) SK-N-SH cells were transfected with WT rod opsin (Rho) alone (control-C) or co-transfected with Rho and ERdj5-HA or ERdj5-SS-HA or ERdj5-H63Q-HA. Twenty-four hours post-transfection, cell lysates were incubated for 2 h with 1D4 antibody against rod opsin (IP: 1D4) or control IgG (IP: IgG), and immunoprecipitated material was blotted with an antibody against HA for detection of ERdj5. Input loading was 12.5% of IP loading. Arrows highlight the ERdj5 immunoreactive band. Asterisks highlight non-specific immunoreactive bands. The position of molecular weight markers, in kDa, is highlighted on the left.
