Manipulation of plant height in garden asparagus (Asparagus officinalis L.) through CRISPR/Cas9-mediated aspSPL14 allele editing

© The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Horticulture Research, 2023, 10: uhad096


Dear Editor,
Reduction in plant height has been associated with yield increases and yield stability in a number of important crop species, such as wheat and rice [1]. In these plants, dwarfing is mainly attributed to the inability to synthesize or respond to certain phytohormones, predominantly gibberellin (GA) [2]. Ideal Plant Architecture 1 (IPA1), an miR156 target gene, encodes SPL14 and it is able to bind directly to the promoters of multiple GA biosynthetic, signal, and deactivating genes in rice [3]. Moreover, IPA1 loss-of-function mutants exhibit dwarf phenotypes [4].
Garden asparagus (Asparagus officinalis L.), known as the 'king of vegetables', is one of the top 10 most popular vegetables owing to its unique texture, taste, and high nutritional value. This species is a diecious and perennial plant with a 1C genome size of 1.3 Gb and 2n = 2x = 20 chromosomes [5,6]. Despite its agronomic importance, it is difficult and time-consuming to modify asparagus traits by traditional breeding due to the diecious nature and the narrow genetic base of the diploid cultivars. The application of biotechnology in asparagus breeding has been lagging due to the lack of tools for efficient genetic manipulation.
The recently characterized clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (Cas9) has been successfully applied to induce site-specific doublestrand DNA breaks (DSBs) for genome editing in numerous plant species [7], but it has never been applied to asparagus. Here, we report successful mutation of the SPL14 allele to produce dwarf asparagus plants using the CRISPR/Cas9 system.
To identify the gene homologous to IPA1 from garden asparagus, a maximum likelihood tree was constructed using 17 SPL asparagus proteins and the rice IPA1 protein with MEGA5 software. The phylogenetic tree showed that AsparagusV1 05.1067 was more closely related the rice IPA1 protein than other SPL asparagus proteins, indicating that AsparagusV1 05.1067 is an IPA1 homolog from garden asparagus (Fig. 1A). Therefore, we named it aspSPL14 in this study. To examine the role of aspSPL14 in the regulation of plant height, we developed a targeted mutation system dependent on CRISPR/SpCas9 in garden asparagus. The 35S promoter-driven SpCas9 was fused to a 3 × FLAG tag and appropriate nuclear localization signals (NLSs), and then an A. officinalis promoter was cloned and applied to express sgRNA, leading to the vector pAspU6-SpCas9 (Fig. 1B).
To determine the ability of SpCas9 to cause mutation in the garden asparagus genome, the editing frequency of SpCas9 in calli was first examined. Three garden asparagus endogenous genes, aspSOFF, aspTDF1, and aspSPL14, were selected as target genes, and four independent sgRNAs were designed to target them (Fig. 1C). A total of 66 transgenic calli were generated by Agrobacterium-mediated transformation, and the target sites were amplified and analyzed using PCR and Sanger sequencing. We found SpCas9-induced editing frequencies ranging from 57.14 to 78.13% across the four target loci (Fig. 1D, Supplementary Data Figs S1-S4), which indicates that pAspU6-SpCas9 successfully produces endogenous gene editing in garden asparagus.
To further validate the contribution of aspSPL14 to dwarfing in garden asparagus, 18 resistant shoots from transgenic calli, including sgRNA3 and sgRNA4, were recovered for the following analysis (Fig. 1D). By sequencing, we found that two T 0 lines separately exhibited homozygous (11.1%) and biallelic (11.1%) mutations at the sgRNA3 target site, while seven of the T 0 lines showed heterozygous (one out of nine) or chimeric (six out of nine) mutations (Fig. 1E, Supplementary Data Figs S5 and S7). The most frequent indel (insertion and deletion) type was deletion (88.97%) at this target site, of which 55.11% were deletions of 1 bp (Fig. 1F). For the mutation efficiency at the sgRNA4 target site, none of the homozygous mutations was observed and only one biallelic (11.1%) mutation was found in nine of the T 0 lines, but eight of the T 0 lines presented heterozygous (one out of nine) or chimeric (seven out of nine) mutations (Fig. 1E, Supplementary  Data Figs S6 and S8). The most frequent indel (insertion and deletion) type was insertion of 1 bp (44.89%) at the sgRNA4 target site (Fig. 1G). Besides introducing indels in the garden asparagus genome, SpCas9 could induce combined indel frequencies of 3.18% (sgRNA3) and 1.39% (sgRNA4) (Fig. 1F and G). The specificity of SpCas9 was also evaluated in garden asparagus. Potential off-target sites of both sgRNA3 and sgRNA4 were analyzed using the online tool CRISPR-GE [8], suggesting that a potential offtarget site for sgRNA3 had two mismatches and the potential off-target site for sgRNA4 contained more than five mismatches. Therefore, the potential off-target site for sgRNA3, AsparagusV1 03.1140 (AspSPL1), was chosen for further experiments and sequencing, which did not reveal any editing events (Fig. 1H). Taken together, ∼89% of deletions were produced by pAsp6-SpCas9 at sgRNA3 target sites, and pAsp6-SpCas9 induced nearly half of the insertions at the sgRNA4 target site.
Next, phenotypic development of plant height was examined in two T 0 lines harboring mutations at the sgRNA3 target site: T 0-1 , with a homozygous mutation, and T 0-2 , with a biallelic mutation, exhibited evidently reduced height compared with the transgenic control plant (Fig. 1I). Moreover, an obvious decrease in plant height was observed in both T 0-11 and T 0-15 , which held chimeric mutations at the sgRNA4 target site (Fig. 1J). Two T 0 lines, T 0-4 , with a chimeric mutation at the sgRNA3 target site, and T 0-18 , harboring a chimeric mutation at the sgRNA4 target site, also presented remarkably decreased height compared with the control (Fig. 1K). These results together prove that aspSPL14 mutation induced by pAspU6-SpCas9 causes a decrease in plant height in garden asparagus.
In summary, we successfully carried out site-specific manipulation of garden asparagus genes using the CRISPR/Cas9 system. In addition, loss of aspSPL14 in garden asparagus was shown to generate mutants with reduced plant height. Tip pruning is a normal farming practice to prevent lodging in asparagus production in China. Considering that semi-dwarf plant architecture can improve lodging resistance of garden asparagus plants, it will be very useful to generate semi-dwarf garden asparagus lines in asparagus breeding. In addition, mutation of IPA1 could affect plant architecture, such as plant height, tiller number, and f lower number [4,9]. Indeed, an increased number of shoots along with plant height reduction was observed in edited transgenic asparagus plants (Fig. 1J and K), and a comprehensive study regarding the effect of aspSPL14 on asparagus shoot development will be performed in the future. More importantly, ∼89% of transgenic plants exhibited no wild-type genome, and clearance of the wild-type genome in the first generation of transgenic lines will be useful for garden asparagus because this species can be propagated in vitro. Our findings demonstrate the applicability of CRISPR/Cas9 in garden asparagus, thereby facilitating both the genetics and the breeding of this important crop species in future.