Electrochemical Approach on a Flavonoid: Miquelianin via Carbon Based Electrodes and Its Analysis from Calystegia Silvatica

Abstract

Background

Miquelianin (MIQ) is a natural phenolic compound found in various plants, including Salvia species, with potential health benefits due to its antioxidant and anti-inflammatory properties.

Objective

This study presents the investigation of electrochemical oxidation pathway and sensitive analysis of MIQ which is isolated from the aerial parts of Calystegia silvatica that grows in Trabzon, Türkiye.

Methods

Electrochemical determination methods have gained significant attention for the quantitative analysis of bioactive compounds due to their simplicity, sensitivity, and cost-effectiveness. The electrochemical determination of MIQ primarily involves the utilization of electrochemical techniques such as cyclic voltammetry and differential pulse voltammetry.

Results

Under optimum experimental conditions, calibration plot for MIQ with a limit of detection of 99.3 × 10⁻⁹ M and 145 × 10⁻⁹ M were obtained using differential pulse voltammetry for GCE and BDDE in the range of 2.0 × 10⁻⁶ to 3.6 × 10⁻⁵ M. The presented method was validated and successfully performed for the determination of the MIQ from plant extracts with excellent recovery values as 104.7, 101.8, and 102.1%.

Conclusion

This study explores the electrochemical oxidation pathway and sensitive analysis of MIQ, isolated from Calystegia silvatica in Trabzon, Türkiye. GCE and BDDE as carbon-based electrodes have been used for the sensitive analysis of MIQ in PB solution at pH 7.0 solution. MIQ was detected by GCE and BDDE with limits of 99.3 × 10⁻⁹ M and 145 × 10⁻⁹ M, respectively, within a concentration range of 2.0 × 10⁻⁶ to 3.6 × 10⁻⁵ M. The validated method demonstrated excellent recovery values of 104.7, 101.8, and 102.1% for determining MIQ from plant extracts.

Highlights

This electrochemical developed method offers promising opportunities for accurate and sensitive analysis of miquelianin. Continued research and technological advancements in this field can contribute to a deeper understanding of the electrochemical behavior of miquelianin and facilitate its practical applications in pharmaceutical, food, and nutraceutical industries, promoting its utilization as a valuable bioactive compound.

Miquelianin, Electrochemistry, Mechanism, Differential pulse voltammetry, Analysis

Accepted manuscripts

Accepted manuscripts are PDF versions of the author’s final manuscript, as accepted for publication by the journal but prior to copyediting or typesetting. They can be cited using the author(s), article title, journal title, year of online publication, and DOI. They will be replaced by the final typeset articles, which may therefore contain changes. The DOI will remain the same throughout.