P4005 Associations of SNPs in hormone-sensitive lipase-like gene 5’ terminal-sequences with fatty acid content in longissimus muscle of Chinese Simmental steers

Abstract

Hormone-sensitive lipase (HSL) is responsible for the hydrolytic action of triacylglycerol (TAG) stored in adipose tissue for free fatty acids (FA) release and considered to be a candidate gene affecting meat quality traits. But the SNPs and function of hormone-sensitive lipase-like gene located at upstream of HSL gene was rarely reported, and its function was not clear. This study detected the 5’ terminal-sequences SNPs of hormone-sensitive lipase-like gene (LOC107131427) (–447 to +111) and its association with FA content in longissimus tissue was analyzed in Chinese Simmental steers. The PCR products of 135 Chinese Simmental steers were sequenced by Sanger sequencing, and five SNPs (c.-862T > C, c.-1002T > C, c.-1609A > G, c.-1781T > C and c.-1821T > C) were found in 5’ terminal-sequences of hormone-sensitive lipase-like gene which had been reported (rs41887429, rs41887428, rs41887427, rs41887426 and rs41887428). The correlation analysis showed that the c.-1002T > C was associated with content of myristic acid, nutmeg oleic acid, palmitic acid, palmitoleic acid, pearl fatty acid, heptadecenoic an acid, stearic acid and oleic acid in longissimus (p < 0.05); the c.-1609A > G was associated with content of myristic acid, palmitic acid, palmitoleic acid, pearl fatty acid, heptadecenoic an acid, stearic acid, oleic acid and arachidonic acid in longissimus (p < 0.05); c.-1781T > C was associated with content of palmitic acid, palmitoleic acid, pearl fatty acid, heptadecenoic an acid, oleic acid and arachidonic acid in longissimus (p < 0.05); and c.-1821T > C was associated with content of myristic acid, palmitic acid, palmitoleic acid, pearl fatty acid, heptadecenoic an acid, oleic acid and arachidonic acid in longissimus (p < 0.05). A haplotype analysis indicated that five SNPs were in linkage and formed three haplotypes: CCGCC (0.733), TTATT (0.225) and TCGTT (0.021). The transcription factor binding sites (TFBS) were predicted by the PROMO and TFBS were changed by SNPs, respectively. The mutant of c.-1821T > C led to an additional binding site for the transcription factor ZF5, and c.-1781T > C led to the elimination of a LIM1, and a C/EBPα TFBS, c.-1609A > G, c.-1002T > C, c.-862T > C led to the elimination of a TBP, a C/EBPα and a HMG I(Y) TFBS in 5’ terminal-sequences, respectively. Our study showed that the SNPs of hormone-sensitive lipase-like gene 5’-terminal sequences may regulate expression levels of hormone-sensitive lipase-like protein which may involve in FA metabolism in cattle muscle.

cattle, hormone-sensitive lipase-like gene, fatty acids (FA)

© American Society of Animal Science 2016