DNA Immunization for Influenza Virus: Studies Using Hemagglutinin- and Nucleoprotein-Expressing DNAs

Abstract

DNA-based immunizations have been used to analyze the ability of DNA-expressed hemagglutinin (HA) and nucleoprotein (NP) to protect BALB/c mice against a homologous influenza virus, A/PR/ 8/34 (H1N1), challenge. The HA DNA, but not the NP DNA, protected mice against the lethal viral challenge. For the HA DNA, single gene gun inoculations of 0.04 μg and boosted inoculations of 0.004 μg of DNA raised complete protection. For the NP DNA, boosted gene gun immunizations of 0.4 μg of DNA and boosted intradermal or intramuscular injections of 50 μg of DNA failed to protect. The protection elicited by the HA DNA vaccine correlated with the titers of neutralizing antibody.

Author notes

*

Current affiliation: Virus Research Institute, Cambridge, Massachusetts.