Southern Plant Diagnostic Network Invasive Arthropod Workshop

The South American rice miner (SARM), Hydrellia wirthi Korytkowski, is a new invasive insect pest of rice in the United States. The species was first described from collections in rice fields from Peru and Colombia. It was reported for the first time in the United States from rice fields in Louisiana in 2004. The species was then reported in different rice areas of Louisiana and Texas. The SARM is a shore fly (Diptera: Ephydridae). The only shore-fly species previously known to infest commercial rice in the US was the smaller rice leafminer, Hydrellia griseola (Fallen). A field survey for the SARM was conducted in the most important rice producing areas of Louisiana and Texas. The objective of the survey was to determine the distribution of this new invasive species and to assess the severity of infestations in rice fields. Efforts were focused to inspect rice fields from one to six weeks of emergence. Commercial rice fields were scouted using a standard 15-inch sweep net. Ten sweep passes at five different locations were performed in each field. Fly adults (dipterans) were collected from nets using a mouth aspirator. Suspicious dipterans were preserved in 70% ethyl alcohol and forwarded to the USDA-ARS-Systematic Entomology Laboratory (SEL) identifier for official confirmation to species level. In addition, suspected damaged plants were taken to a laboratory and fly larvae reared until adult emergence. Fly adults and associated parasitoids emerged in the laboratory were shipped in vials containing 70% ethyl alcohol. A total of 15 different Louisiana parishes were surveyed which included 1223 hectares (3023 acres) of rice. The following parishes were surveyed in 2005: Vermilion, 260 hectares (642 acres); Acadia, 191 hectares (471 acres); Lafayette, 12 hectares (30 acres); St. Landry, 85 hectares (210 acres); Jefferson Davis, 168 hectares (414 acres), Allen, 103 hectares (254 acres); Calcasieu, 45 hectares (110 acres); St. Martin, 81 hectares (200 acres); Cameron, 81 hectares (200 acres); Evangeline, 12 hectares (30 acres); Avoyelles, 36 hectares (90 acres); Concordia, 133 hectares (328 acres); Tensas, 3 hectares (7 acres); Franklin, 2 hectares (5 acres); East Carroll, 13 hectares (32 acres). Forty five vials containing suspicious fly samples (including 5 vials with emerged parasitoids) were sent to the SEL identifier for identification to species level. The field survey revealed that the SARM is widely distributed in all the important rice producing areas of Louisiana. Higher infestations, i.e. those causing significant yield losses, were observed in coastal parishes including Cameron, Jefferson Davis, Acadia and Vermilion parishes. The insect was found at very low levels in other rice areas of southwest, central and northeastern Louisiana including Calcasieu, Allen, St. Martin, Concordia and Tensas parishes. The distribution of the species in Texas counties includes Calhoun, Colorado, Jackson, Jefferson, Matagorda, and Wharton counties.

Twenty-eight species of Heteroptera in five families are discussed and considered as potential or actual pests of economic concern in the United States. The five families are Coreidae, Lygaeidae, Pentatomidae, Scutelleridae, and Tessaratomidae. Three leaf-footed bugs of economic interest within the family Coreidae are Leptoglossus australis (F.), L. occidentalis (Heidemann), and L. zonatus (Dallas). Leptoglossus australis is the only species of Leptoglossus that does not occur in the Western Hemisphere. The other two bugs are native to Mexico and the western US. Leptoglossus occidentalis has been detected in the northeast and has been steadily moving south. The second species, L. zonatus, has already been found in Florida and other southeastern states. The family Lygaeidae, known as seed bugs, contains several species of concern to agriculture in the southeast. Dieuches armatipes (Walker), a pest of peanuts in Africa, has been in Florida for several years. Oxycarenus hyalinipennis Costa, a cotton pest, is known from every continent except North America. It has recently been detected in the Bahamas. Other species of concern include Blissus antillus Leonard from the Caribbean, Dimorphopterus gibbus (F.) from Africa, Asia and Australia, Nysius huttoni Buchanan-White from New Zealand, Nysius vinitor Bergroth from Australia, and Spilostethus pandurus (Scopoli) from Africa, Asia and Europe. All of these species have shown potential to expand their ranges in recent years. Pentatomids, or stink bugs, that are already in the US and may spread in the southeast are Euschistus quadrator Rolston from Mexico and Texas now in Florida and Louisiana; the Asian species Halyomorpha halys Stål now in Pennsylvania and New Jersey; and Oebalus ypsilongriseus (De Geer) from South and Central America now in Texas and Florida. A serious rice pest that has moved close to the US is Tibraca limbativentris Stål, the stem rice stink bug, native to South America; it has been found in the Caribbean. Other exotic species of concern include Dichelops melacanthus (Dallas), D. furcatus (F.), Edessa meditabunda (F.), Euschistus heros (F.), Loxa deducta Walker and Oebalus poecilus (Dallas), all from South America; Aelia acuminata L. from Europe and Asia; Aelia rostrata Boheman from the Middle East; Biprorulus bibax Breddin from Australia; and Scotinophora lurida (Burmeister) from Asia. Eurygaster integriceps Puton, a shield bug (family Scutelleridae), is considered to be among the world's worst agricultural pests. It feeds on cereal crops, including wheat, throughout its native range in Asia and the Middle East. Although these crops are not abundant in the southeast, early detection of this pest would be critical. Musgraveia sulciventris Stål, known in Australia as the Bronze Orange Bug, is a large tessaratomid that damages citrus trees. Each year, hundreds of exotic insect species approach the United States through international trade and travel. As indicated by pest interceptions made by federal and state inspectors at ports of entry, nurseries and other locations, these potential invaders reflect nearly the entire spectrum of plant pest taxa including obscure species and well documented pests. Among pests of especially high concern, we discuss two in this presentation: Helicoverpa armigera (Hubner) and Anoplophora chinensis (Forster). Respectively, these species represent exotic pests which arrive with imported consumption products (especially cut flowers) and propagative material (especially bonsai plants). Several other insects which arrive as hitch hikers with non-host commodities are highlighted and their pathways discussed. Criteria used to designate high priority pests are noted and illustrated for each insect; relevant pest interception data are provided and evaluated; and, mitigation measures specific to each of these pests and their pathways are described. Increases in the volume and efficiency of international trade and travel have significantly increased the risk and incidence of unintentionally introducing invasive plant pests to the United States. The exact means by which any given adventive species has entered the U.S. is rarely determined. However, patterns of pest entry become evident upon examining pest interception records resulting from inspections of imported commodities from commerce and travelers. Pathways for pest introduction, particularly as defined as a commodity from an origin, present varied degrees of pest risk and require different levels of effort to effectively exclude pests. In this presentation, I review summaries of APHIS, PPQ pest interception data from 1984-2000 reported by McCullough et al. (2006) to characterize major pest pathways. Additionally, I briefly discuss certain high risk pest pathways, provide insight on the strengths and limitations of pest interception data for evaluating pathway risk, and outline federal interagency efforts to assess relative pathway risk.

Pathways of Introduction and Pest Risk Assessment Issues for Exotic Pests of Regulatory Significance
USDA, APHIS, PPQ safeguards agriculture and natural resources from the risks associated with the entry, establishment, or spread of animal and plant pests and noxious weeds. To carry out this mission, communication channels must be established with stakeholders to deal with exotic pest detections in a timely manner from diagnostic and regulatory perspectives.
PPQ has traditionally worked closely with the State Departments of Agriculture, in which designated State Plant Regulatory Officials (SPRO), work hand in hand regarding newly detected exotic plant pests within their respective state. From a diagnostic standpoint, PPQ has its own domestic and port-of-entry identifier specialists that include entomologists, plant pathologists, botanists and mollusk experts. With the continual need for identification services, PPQ has broadened its diagnostic services reach to university and private laboratories in order to provide timely response to exotic plant pest diagnoses. With the development of the National Plant Diagnostic Network, this additional exotic pest identification resource has increased the ability to identify exotic plant pests of concern to PPQ and it's stakeholders on a broader scale. After proper identification channels have been followed, final exotic plant pest identification confirmation must be completed by the USDA, ARS Beltsville, MD Laboratory. Regulatory action(s) will be cooperatively determined with the SPRO in the affected state or states in partnership with PPQ. Such actions, based on a through review of the risk associated with a particular exotic plant pest, could involve the establishment of a quarantine area, treatments and/or regulatory actions affecting the movement of infested or infected host material from the established area. The key to successfully addressing new exotic plant pest introductions is open communications between PPQ and cooperators. There is a vital need for such communication to be timely so that needed actions can be implemented in an effective manner. The about 5500 recognized species of Thysanoptera are classified into about 750 genera and 9 families. Thrips occupy widely disparate niches with a diversity of lifestyles. Thrips are small, opportunistic insects with cryptic habits and can be stealthy insect invaders. The movement of plant species in international trade for over two decades has resulted in the emergence of numerous thrips species as major invasive pests causing extensive crop damage, vectoring of viral diseases, and destabilizing of integrated pest management systems. Over 20 species of thrips are now cosmopolitan. Frankliniella occidentalis invaded the southern USA in the 1980's. This is the key vector of Tomato spotted wilt virus, the key disease of many ornamental, vegetable, and agronomic crops in the region. Thrips palmi is a highly polyphagous pest species that became established in southern Florida in the 1990's. Scirtothrips dorsalis is emerging in many parts of the world. It is established in the landscape in Florida where it has already become a major pest of numerous ornamental and vegetable crops. A Megalurothrips species recently was established in the southern USA. Numerous other species are invasive in the geographic region, and many species of thrips will emerge as quarantine practices change and the pace of international commerce increases. Early recognition is a challenge. Few specialists are trained in rapid and accurate identification of invasive pest species of thrips. An overview of the morphological characters for identifying thrips to family, genus, and species will be presented that focuses on the important invasive thrips species. Currently, an interactive identification system and information system that provides a fully illustrated and user-friendly means of recognizing economically important thrips is available. Similar identification and information systems are being developed specifically for the southern USA. dominant turf white grub species on Long Island, New York) as well as nursery stock: the grub stage feeds on and damages roots, sometimes killing host plants or areas of turf. Both field-and container-grown nursery stock can be infested. In New York, common woody hosts include rhododendron, azalea, andromeda, junipers and hemlock, and occasional broadleaf species such as cotoneaster. European woodwasp (Sirex noctilo) was found in landscape pines near Fulton, New York in September, 2004 and subsequent surveys have documented its presence in much of central New York and a few counties in northern Pennsylvania. It attacks live pines, unlike other native woodwasps, and is associated with the death of pines in Australian and New Zealand plantations where the insect has been introduced, causing up to 80% mortality. Eastern Massachusetts has been battling an invasion of winter moth (Operophtera brumata) for at least 15 years. This insect feeds on a wide range of deciduous hosts, such as crabapple, apple, basswood, ash, maples, oak and some spruces. Repeat defoliation of forest and ornamental plants is a concern, but the larvae also feed on very young buds which may threaten blooms and fruit crops such as apple, cherry and blueberry. Portions of the region have sustained heavy defoliation for several years in a row, including an estimated 34,000 acres in Massachusetts in 2005. Winter moth has now spread to new areas in southern New England. Viburnum leaf beetle (Pyrrhalta viburni) has spread eastwards from Canada to New England and south into northern Pennsylvania and northeast Ohio. Both adults and larvae feed on and can seriously defoliate and kill a wide variety of viburnum species, including both introduced ornamentals and natives, although some appear to be tolerant or resistant. Japanese cedar longhorned beetle (Callidiellum rufipenne) is originally from Asia, but has clearly become established in parts of the northeastern US. In 1998 it was found infesting arborvitae in a Connecticut nursery and subsequent surveys showed it to be established around southern New England, Long Island, New York and New Jersey. Both live and dead plant material have been found infested. Hosts include plants in the families Taxodiaceae and Cupressaceae. Christmas tree and conifer plantations are on alert for common pine shoot beetle (Tomicus piniperda) which has spread west, south and east throughout much of the north central and northeastern US, extending as far south as West Virginia. As a quarantine pest, movement of host material from or through affected areas is subject to restrictions. It breeds in dead or dying pines and occasionally other conifers. The adults tunnel into lateral shoots of host plants, causing twigs to die back or break at the point of entry. The damage can spoil the appearance of trimmed Christmas trees or other conifers and in heavy infestations can reduce tree growth. Scots pine is among the most preferred, but Austrian, eastern white, red and jack pine are also hosts. Brown marmorated stinkbug (Halyomorpha halys) may be a greater concern in tree fruit, in soybeans and as a household invader, but it can also damage ornamental plants. The adults and nymphs feed on foliage, causing a whitish blotching which might reduce aesthetic value and marketability. This Asian species was believed to be in the Allentown, Pennsylvania area at least since 1996 and has now been found in parts of southeastern Pennsylvania, New Jersey, Maryland, West Virginia, South Carolina and Oregon. European crane flies (Tipula paludosa and T. oleracea) are reported in parts of the Pacific Northwest, western Canada and New York. The larvae, known as leatherjackets, damage turfgrass (esp. T. paludosa), and are also known as pests in pastures and occasionally in greenhouses and nurseries, feeding on stems and roots of container-grown conifer seedlings. Goals of NPDN exercises include: a) helping all exercise participants (local, extension, state and federal) understand their roles and responsibilities, and how their efforts coordinate with those of the others while practicing standard operating procedures (SOPs) in a non-critical environment, and b) improving the SOPs by identifying and removing ambiguity in the protocols as well as gaps in the procedures. An exercise is composed of three phases: 1) a pre-exercise training session via conference call for all participants, 2) the exercise scenario where participants use the NPDN exercise SOP, 3) a post-exercise debriefing session via conference call to evaluate the exercise and the NPDN exercise SOP. The Plant Diagnostics Information System is a secure website module for monitoring and documenting exercise activities. The website provides critical contact information for the participants, a copy of the SOP to be exercised, an activity log, goals and objectives of the exercise and the photo sheet file that is used as a "plant sample". The exercise module has been used for all NPDN exercises conducted to date. The impact of these exotic scale insects is not completely known for all of the species in the south but the biggest impact can be seen with the cycad Aulacaspis scale and pink hibiscus mealybug. In each of these two cases, the most common hosts were relatively free of any major economic pests. After the introduction of the pests, costs of control and economic losses increased to unacceptable levels. Detection and recognition of new pests is paramount now more than ever with the increase in population movement and increase in the ornamental plant industry. The possibility of new exotics finding their way into the southern United States is high. The Cooperative Agricultural Pest Survey (CAPS) program has maintained scale insects on their survey list. The list has incorporated species from four families; Coccidae, Diaspididae, Margarodidae and Pseudococcidae. One of the mealybugs on the list is the passionvine mealybug which was thought to have been found in California in 2006. This resulted in an a more thorough look at current keys available for the determination of this mealybug. The pink hibiscus mealybug (PHM) (Maconellicoccus hirsutus (Green)) is considered as a major economic pest for many crops grown throughout its range. It is believed to be native to the Orient and is known from over 300 host plant species. It was first found in the Western Hemisphere in Grenada in 1994. With PHM having such a wide host range and being considered as an economic pest, many scientists from the USDA, FDACS-DPI, land grant Universities and from many of the neighboring Caribbean islands visited the Grenada infestation. After viewing the extent of damage from the infestation, a preintroduction strategy started with educational materials States and all but one is listed as occurring in the southern region. Species level identification can be difficult for the wax scales and no one concise key exists that addresses both field and slide mounted characteristics for the species found in the United States. In addition to the thirteen species that are present in the United States, there are two species of Ceroplastes that are currently on the USDA-CAPS survey list, they are: Ceroplastes destructor Newstead (the soft wax scale) and Ceroplastes japonicus Green (Japanese wax scale). Both of which superficially resemble species that occur in the United States. Accurate field identification for these two species is generally not possible. In these cases, slide mounted keys are needed. This presentation presents both tentative field and slide mounted keys for identification of the thirteen species of Ceroplastes occurring in the United States.  thrips, have a wide host plant range, which includes many commonly grown ornamentals, and pose a significant threat to agricultural crops. In addition, little is known about biology of most of these pests or their potential geographic range. Although it has not been detected in the United States yet, the red palm mite (Raoiella indica Hirst) is expected to arrive shortly.

Arthropod Pests New to South
The Japanese beetle, Popillia japonica, was first found in the U.S. at a Riverton, New Jersey nursery just outside of Philadelphia, Pennsylvania in 1916. It was likely brought in with the soil around Japanese iris bulbs sometime before such items were inspected under the Federal Plant Protection Act of 1912. Attempts were made to eradicate the infestation, but little was known about the beetle since it was relatively rare in its natural range from northern Japan up into Siberia. Cool temperatures, lack of grassland, and effective parasitoids kept populations at low levels there. Reports of the Japanese beetle in Korea and China have proved to be mistaken identities with some of the many Popillia species found there. In addition to our infestations, the beetles have become established in three of the Portuguese islands of the Azores, and Ontario and Quebec in Canada. All states touching, and east of the Mississippi River, are now likely infested. In addition, Missouri, Kansas, Nebraska, Arkansas, Texas, Oklahoma, Colorado, New Mexico, and Oregon have various population densities. Although only 0.5 sq. miles in New Jersey were infested in 1916, this increased to 5,700 in three states in 1930, 20,611 in eight states in 1941, and 76,504 in 15 states by 1952. Beetles were first found in Missouri in 1949, but that population remained small and isolated in Saint Louis for nearly 50 years before the beetle moved throughout the state. Quarantine programs helped to slow the beetle's spread, but did not prevent it. Two converging events in the 70s have lead to a jump in infested states since that time. First the use of DDT and chlorinated hydrocarbons, which had been very effective in long term control for quarantine programs against Japanese beetle larvae in the soil environment, was withdrawn from the market. This left more toxic phosphates and carbamates which gave weeks or months of control in the soil rather than years. Also then a majority of the states became infested, and a National Japanese Beetle Quarantine was no longer supported. The National Quarantine was replaced by variety of state imposed quarantines and restrictions on the movement of nursery plants and soil. To achieve uniformity in treatments and shipping requirements, a Harmonization Agreement was established to facilitate shipment of nursery stock to western states. This agreement prevented shipment of nursery stock with soil to California, Washington, and Oregon, but allowed shipments to other states following specific treatments. However, no treatments were available which would remove all Japanese beetle larvae from the nursery stock and the beetle rapidly expanded its range to the west. Eradication programs have removed isolated infestations of the beetle as early as 1944 in Novia Scotia, during the 60s, 70s and 80s from three locations in California, and more recently in Nevada and Oregon. The ability of Japanese beetle lures were an intrigal part of the eradication programs by delineating the size and distribution of the populations. Today, many "greener" pesticides such as neonicotinoid compounds and growth regulators are used for control.
These materials have been adopted for larval control by home owners and turf managers, but their value in eradication programs is an open question. The Cooperative Agricultural Pest Survey (CAPS) program is a collective effort by Federal and State agricultural organizations to conduct surveys for exotic pests and biological control agents. CAPS surveys can be thought of as our first-line of defense for detecting exotic pests once the cargo has passed through our borders. As such, it is of key importance that survey efforts target pests that have the potential to cause great economic damage while not known to occur (or of limited distribution in) the United States. To this end, the CAPS program strives to find new pests early (prior to establishment) and respond rapidly to any finds so that they can be eradicated before they become widely established. The key to the CAPS program is its cooperative nature. PPQ establishes partnerships with state departments of agriculture and universities, which through our common goal of early detection, allows us to pool our resources to survey for exotic pests as efficiently and economically as possible. PPQ and our cooperators use two major tools to help in our survey efforts. First, trap locations are determined based on risk. Data (such as pest interception records, climate, host material, etc.) are used to narrow down the possible pest locations in order to place traps in the areas of highest risk. The other tool is public outreach. An aware public can be a valuable tool for detecting exotic pests and mitigating their spread by knowing who to contact if they find something suspicious.

Developing a Pest List and Image System to Support the SPDN
The Bugwood Network (www.bugwood.org) has partnered with The Southern Plant Diagnostic Network (SPDN) to develop a comprehensive list of organisms-ofinterest to SPDN. This list is being used to solicit images to populate the IPMImages image archive system (www.ipmimages.org) to support SPDN training and educational programs. This project builds upon the successful Bugwood Network image system that provides high resolution, identified and credited images that are available at no-cost for educational uses. The Bugwood image system currently contains more than 54,000 images on 9,000 subjects that have been taken by over 1,100 contributors in 45 countries. Bugwood web sites received 118 million hits during 2006. The Bugwood Network -SPDN partnership has been made possible through a CSREES Southern Region IPM project with objectives to: 1) identify the species of concern to the SPDN, 2) obtain a comprehensive set of images of the listed species including all life stages and depictions of damage and 3) provide an interface to easily access these images as well as others to be used in the SPDN training and education modules. The list includes native and nonnative pests as well as biological controls found in the Southern region. It also includes exotic species that they are not currently present, but may pose a significant threat if introduced. The end result will be a useful tool to aid diagnosticians and a reliable source of quality, high-resolution images for anyone creating publications, presentations and other educational materials. The organisms-of-interest list contains 252 insects and 96 pathogens and is available at www.ipmimages.org/spdn. It is categorized by pest status (exotic, native, introduced), commodity and the number of images available for each life stage. Project personnel are soliciting image contributions for each species to include whole-organism images of each life stage, damage symptoms, as well as diagnostic features and characteristics. All images obtained through this project will be incorporated into and made available for SPDN and other educational and training needs through IPMImages.

Laurel Wilt: A New Disease Threatening Redbay and Other Plants in the Lauraceae
Identification characters and techniques for Lepidoptera are discussed. Eggs are slide mounted to examine the micropyle and chorion. Preserved larvae of leaf miners should be associated with the pressed mine of the host. Larvae are studied by slide mounting the body and mouthparts. Pupal identification is more accurate if the larval skin and cocoon are not discarded. Submit cast pupal skins dry. If the pupal eye is dark, there is a good chance the genitalia of the pharate adult can be dissected and examined. Immatures are best reared to adult; this is especially true of eggs. Sticky traps should never be sealed in plastic bags, folded one on top of the other, or sealed with heavy duty staples. Instead, fold them in a triangle when viewed in cross section, then close with weak scotch tape. Screen samples and only forward those closely related the target. Traps should be labeled with locality data, a unique code number, and the target pest for the survey. A "mini pest risk assessment", using criteria I developed as modified by members of the Northeast Exotic Pest Survey Committee in 1992, was applied to Lepidoptera of regulatory concern to the southern United States. The criteria were: tolerance to the southern US climate, host availability, survey methodology, ease of identification, availability of a high hazard site, potential economic impact, and an evaluation of the entry and establishment potential of the pest. Lepidoptera on corn, sorghum, cotton, citrus, sugarcane, soybean and ornamentals were favored. Two categories of pests were noted (threats and CAPS targets). A threat is defined as a species with a negative impact to the environment, trade, or the agroecosystem. A CAPS target should also be practical to identify, be frequently intercepted or have a good pathway to enter the USA, and have a high probability of establishment after arrival. Selected pests meeting these criteria that either are not in the United States, or are of limited distribution, include: Opogona sacchari (Tineidae); Phyllocnistis citrella (Gracillariidae); Blastobasis graminea (Coleophoridae); Pectinophora scutigera, Platyedra subcinerea (Gelechiidae); Acrolepiopsis assectella (Acrolepiidae); Prays citri (Yponomeutidae); Synanthedon myopaeformis (Sesiidae); Pryeria sinica ( Since its discovery in Southeast Michigan in 2002, the emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), appears to be living up to expectations and predictions about its potential spread and destruction of ash trees, Fraxinus spp., in North America. Currently, the generally infested "core" area includes 21 counties in Southeast Michigan and extends into Ohio and Ontario, Canada. In addition, numerous outlier infestations have been found throughout Michigan's Lower Peninsula, Ohio, Indiana, and Ontario. An outlier in Maryland that originated from infested Michigan nursery stock was first detected in 2003 and is still under eradication. Two isolated infestations were detected in the Chicago area of Illinois in the summer of 2006. As of 1 December 2006, APHIS revised the federal quarantine to include the entire states of Ohio, Indiana, and Illinois, in addition to the Lower Peninsula of Michigan. State quarantines further restrict within state movement of ash. The area infested with EAB now exceeds 40,000 square miles in Michigan, Ohio, Indiana and Ontario and it is estimated that the beetle has killed more than 20 million ash trees in the core infested area of the US. Early detection of emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), remains a major challenge for regulatory officials due to the delay in onset of visual symptoms of attack. Currently, regulatory and resource management agencies rely on girdled trap trees for statewide survey and detection programs. However, locating suitable detection trees can be difficult and felling and debarking trees to locate galleries is labor intensive and costly. Development of effective traps and attractants is a high priority for the EAB management program. Previous studies suggest that A. planipennis is attracted to the color purple, to blends of host volatiles from ash bark and foliage, and to girdled ash trees. It has also been found that A. planipennis prefers to attack trees with rough bark. We evaluated attraction of A. planipennis to triangular purple panel traps baited with various combinations of ash volatiles that elicit antennal responses by A. planipennis as well as volatiles from ash that have behavioral activity in other bark or wood-boring insects. We also evaluated attraction of A. planipennis to traps that incorporated multiple components of attraction. Multicomponent traps included triangular purple panels mounted at 1.5 and 2.5 m on a purple pole. The upper and lower panels were baited with foliar and bark volatiles, respectively. Panels were also coated with a rough "bark" texture. Treatments included binary and ternary combinations of foliar volatiles, bark volatiles, and texture. Overall, we captured more than 4,000 A. planipennis on the 160 multi-component traps used in the experiment. Traps baited with both the foliar volatiles and bark volatiles but without texture captured the most A. planipennis. Traps baited with the bark volatiles alone and coated with the texture caught significantly fewer A. planipennis than traps baited with both bark and foliar volatiles but without texture. Traps coated with texture and baited with either the leaf volatiles alone or the leaf and bark volatiles caught an intermediate number of A. planipennis. The combined effect of omitting the leaf volatiles and including texture significantly reduced attraction compared to traps with both the leaf and bark volatiles but no texture. Therefore, it appears that leaf volatiles are an important factor in A. planipennis attraction. The texture that was applied to the traps may have interfered with A. planipennis capture, due to reduced adhesion of the beetles to the rough surface which was difficult to coat with Pestick. Thus, bark roughness is probably not an important factor in long range attraction of A. planipennis, but is more likely involved in post-landing acceptance of oviposition sites. The results will lead to improved trapping and detection methods for A. planipennis.

Non-native Forest Pests of Concern
ecological impact is on the plant and animal communities is unknown, they do speed the breakdown of the detritus. Exotic earthworms (Class Oligochaeta) on the other hand cause tremendous ecological damage to soils and their communities. They are similar in that they now dominate most soil communities and are also found in very high populations. They have even changed soil structure so drastically that the soil belongs to a different order following invasion, and have led to localized extinctions of plant species. Their ecological impact affects all plant and animal species, including increasing wildlife species that feed on earthworms. Support in part comes from the National Park Service R5240050019 [CESU-CU COSW].