In Vitro Viral Transformation off Chemically Transformed Cells

Summary

Hamster embryonic cells grown in a suspension of 3-methyl-cholanthrene underwent neoplastic transformation. These cells, when injected into homologous hosts, could produce tumors, but morphologic change in vitro was not significant. When these transformed cells were exposed to simian virus 40 or the LLE 46 strain of adenovirus 7 (the SV40-adenovirus 7 “hybrid”), they were further transformed with altered morphologic and growth characteristics in vitro, chromosomal changes, appearance of intranuclear SV40 T antigen, and changes in the morphology of tumors produced by injection of cells into homologous hosts.