Detection of Antigens of a New Diethylnitrosamine-Induced Transplantable Hepatoma by Delayed Hypersensitivity

Summary

Strain-2 guinea pigs, immunized with a syngeneic transplantable hepatoma, develop a specific skin reaction to an intradermal inoculum of tumor cells derived from the hepatoma. The rate of development, onset, duration, histologic features, and specificity of this skin reaction were characteristic of delayed hypersensitivity in guinea pigs. The living tumor cells, used to provoke the skin reactions, multiplied and formed intradermal papules. Tumor papules in immunized animals with positive skin tests were smaller and regressed faster than those in untreated animals. Immunization with another strain-2 tumor or normal tissues did not influence papule size.