Antigenic Studies on Mouse Lymphomas Bearing the T190 Translocation Chromosome

Summary

Heterozygous mice of the T190/tf translocation stock, having one superlong and one short marker chromosome, involving the H-2-bearing ninth linkage group, were typed by membrane fluorescence and cytotoxicity tests against a panel of H- antisera. Marker-positive segregants differed serologically from their marker-negative siblings. Lymphomas were induced by the Moloney virus in (A.CA × T190/tf)F₁mice. A combined serological and cytogenetic analysis showed that the tumors could be subdivided into two main classes, corresponding to marker-positive and marker-negative types. Each lymphoma was relatively stable with regard to karyologic and correlated antigenic characteristics during serial passage in (A.CA × T190/tf)F₁ mice. This system opens new possibilities to study the genetic mechanisms of H-2 antigenic variation, demonstrated previously in heterozygous tumors.