Properties of Arginase From SV40- Induced Hamster Fibrosarcomas and Hamster Liver Tissues

Summary

Arginase was partially purified and characterized from SV40-induced fibrosarcoma and hamster liver tissues. The level of arginase activity was elevated in the fibrosarcoma tissue. Two fractions containing arginase activity were obtained from both tissue sources by chromatography on CM-cellulose columns. The molecular weights of these fractions were determined. Fractions I and II from the same tissue exhibited essentially identical Michaelis constants for arginine, as well as similar inhibitor constants for ornithine and canavanine. However, differences were noted when Michaelis and inhibitor constants of fibrosarcoma and hamster liver tissue were compared.