Intercellular Transfer of Toxic Components After Laser Irradiation

Summary

The application of heat shock and ruby laser treatment of unstained cells, and argon laser treatment of acridine orange-photosensitized cells showed a loss of intact lysosomes, with the use of the Gomori acid-phosphatase staining reaction. Primary tumor cells, established within a 24-hour period in tissue culture, also were irradiated with an argon laser microbeam after acridine orange photosensitization. The irradiated cell demonstrated zeiosis and nuclear pycnosis which, in a few cases, showed a transmission of toxic products into adherent neighboring cells. The frequency of transmission ranged from 15–45% in most of the tumor cell types, but only 0–5% in nonmalignant cell types.