Immunologic Cross-Reactivity Between Antigen of Unfertilized Mouse Eggs and Mouse Cells Transformed by Simian Virus 40

Summary

Antisera against one-celled unfertilized mouse eggs, against PF-1 cells [C57BL fibroblasts transformed by simian virus 40 (SV40)], and against C57BL spleen cells were produced in guinea pigs. The antiegg (AE) serum was cytotoxic at a higher dilution for SV40-transformed mouse cells but not for SV40-transformed cells of other species. AE serum was cytotoxic for mouse cells transformed by viruses other than SV40 only at low dilutions. The cytotoxic effect of AE serum against mouse eggs was eliminated only by absorption with mouse cells transformed by SV40 and not by mouse cells transformed by other viruses. The anti-PF-1 serum was cytotoxic for mouse eggs and PF-1 cells. Absorption of this serum with C57BL spleen cells eliminated the reaction against spleen cells, but had no effect on the reaction against PF-1 cells, SV40-transformed cells of other species, and mouse eggs. Antispleen serum had no effect against mouse eggs and only reacted against PF-1 cells in low dilution.