Brief Communication: Proliferation of Preneoplastic Mammary Nodule Outgrowth in Mammary Fat Pads of BALB/c Mice in Organ Culture

Summary

The whole “gland-free” inguinal (fourth) mammary fat pad of BALB/c mice containing a fragment of mammary nodule outgrowth transplanted 3 weeks earlier was incubated in hormone-supplemented medium. The nodule tissue responded to the growth-promoting action of the hormones and, after incubation for 12 days in organ culture, filled over 50% of the fat pad. However, when transplantation was done at the time of explantation, subsequent growth in vitro was not observed.