Cytogenetic Studies on the Mechanism of Formation of Isoantigenic Variants in Somatic Cell Hybrids. I. Banding Analyses of Isoantigenic Variant Sublines Derived From the Fusion of TA3Ha Carcinoma With MSWBS Sarcoma Cells

Summary

The MSWBS sarcoma, an isoantigenic loss variant selected from a near-diploid heterozygous tumor, lost one ofthe parental H-2 antigens by a mechanism that did not involve the loss of a whole chromosome bearing the relevant genetic determinant. But when the same selective procedures were used to select isoantigenic loss variants from hybrid cell tumors, loss of one parental H-2 antigen was associated with elimination of both chromosomes bearing that genetic determinant. This was established in an especially favorable hybrid line, TA3Ha/ MSWBS, in which both of the #17 chromosomes bearing the H-2 determinants derived from TA3Ha were morphologically normal, and both of the #17 chromosomes derived from MSWBS were involved in identifiable translocations.