Radiolabeled Antitumor Antibodies. I. Antibody-Specific and ImmunoglobulinSpecific Binding Sites on Moloney Lymphoma Cells (LSTRA)

Nord, S.; Weissman, I.L.

doi:10.1093/jnci/53.1.117

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Radiolabeled Antitumor Antibodies. I. Antibody-Specific and ImmunoglobulinSpecific Binding Sites on Moloney Lymphoma Cells (LSTRA)

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Summary

We used a paired, radiolabeled immunoglobulin technique to showthat LSTRA Moloney lymphoma cells may bind rat IgGa immunoglobulins at either antibody- specific (Fab) or immunoglobulin-specific (Fe) portions of the IgGa molecule. Fe-specific binding is inhibited in the presence of normal rat serum (NRS), whereas Fab-specific binding is not. Sodium dodecyl sulfate-polyacrylamide gel analysis of eluted radiolabeled anti-Moloney proteins (bound to LSTRA cells in the presence of NRS) revealed that the bound proteins were indeed immunoglobuli.

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Radiolabeled Antitumor Antibodies. I. Antibody-Specific and ImmunoglobulinSpecific Binding Sites on Moloney Lymphoma Cells (LSTRA)

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Radiolabeled Antitumor Antibodies. I. Antibody-Specific and ImmunoglobulinSpecific Binding Sites on Moloney Lymphoma Cells (LSTRA)

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