Extraction of Gross Surface Antigens From AKR Mouse Lymphoma Cells With Potassium Chloride

Brandchaft, Phyllis B.; Boone, Charles W.

doi:10.1093/jnci/53.4.1079

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Extraction of Gross Surface Antigens From AKR Mouse Lymphoma Cells With Potassium Chloride

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Summary

Approximately 47% of the surface antigen activity present on Gross virus-infected AKR mouse lymphoma cells was extracted in soluble form with 3M potassium chloride, as measured by inhibition of radioiodinated antibody binding to surface antigens. After precipitation with 60% ammonium sulfate and Sephadex chromatography, the soluble antigen was purified 6.4-fold, with a total recovery of 13% of the activity initially detected on the whole cells. The antigen elicited productiol of antibodies against Gross surface antigens in allogeneic mice.

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Extraction of Gross Surface Antigens From AKR Mouse Lymphoma Cells With Potassium Chloride

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Extraction of Gross Surface Antigens From AKR Mouse Lymphoma Cells With Potassium Chloride

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