Relationship Between the Cellular and Viral Antigens of a BALB/c Myeloma and Murine Leukemia Virus

Summary

FLOPC-1 murine myeloma and murine leukemia virus (MuLV)-producing normal rat kidney cells (NRK-MuLV) were found to possess common antigens. The relationship wasdetermined by use of rabbit, rat, or mouse antisera, generated against FLOPC-1 tumor cells, MuLVmurine sarcoma virus, MuLV-Kirsten, or FLOPC-1 C particles, in immunofluorescent or complement-fixation assays. While FLOPC-1 myeloma cells shared similar antigens with MuLV and NRK-MuLV cells, they also appeared to possess unique viral and cellular antigens. One viral antigen was in the nucleus of myeloma cells and the other appeared to be in the cell membrane. The distinct FLOPC-1 myeloma cellular antigen also appeared to be in the cell membrane.