Brief Communication: Mouse Mammary Tumor Virus Polypeptide Precursors in Intracytoplasmic A Particles

Summary

Intracytoplasmic A particles were analyzed by immunodiffusion and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) before and after enzymatic cleavage with trypsin. A common antigen in A particles was detected by antisera prepared against purified intracytoplasmic A particles, purified mouse mammary tumor virus (MMTV), and a purified MMTV core polypeptide (p28). Despite this correiation, no SDS-polyacrylamide band migrating at p28 was observed in purified intracytoplasmic A particles. However, after incubation with trypsin, A particles subjected to SDS-PAGE produced only two polypeptide bands. They were observed at p28 and p15-10. Ouchterlony analysis of the trypsin-cleaved A particles revealed no alteration in the antigenicity of the particles. These results suggested that some structural components of intracytoplasmic A particles are polypeptide precursors of MMTV core proteins.