Estrogenic Properties of 3,9-Dihydroxybenz[a]anthracene, a Potential Metabolite of Benz[a]anthracene

Abstract

The estrogen receptor binding properties of 3,9-dihydroxybenz[a]anthracene (3,9-diOHBA) were determined in uterine cytosol of immature Sprague-Dawley rats by competitive binding experiments with (³H]estradiol and sucrose density centrifugation. 3,9-DiOHBA inhibited estradiol binding to the 8S binding protein at a concentration (1.2×10⁻⁵M) approximately equal to that of nafoxidine-HCI (3×10⁻⁵M) required to inhibit estradiolspecific binding, and bioassay for estrogenic activity substantiated this finding.