Virus Isolation Test for Feline Leukemia Virus

de Noronha, F.; Poco, A.; Post, J. E.; Rickard, C. G.

doi:10.1093/jnci/58.1.129

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Virus Isolation Test for Feline Leukemia Virus

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Abstract

A method was developed for detecting feline leukemia virus (FeLV) infection in cats by coculturing separated blood lymphocytes and platelets with clone 81 cells carrying a murine sarcoma genome but free of leukemia virus (S+L-). FeLV caused cell transformation that was easily visible 3–12 days after infection. This method is simple, sensitive, and easily read; it has the advantage of directly detecting infectious FeLV rather than viral antigens.

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Virus Isolation Test for Feline Leukemia Virus

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Virus Isolation Test for Feline Leukemia Virus

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