Isolation of T-Lymphocytes From Disaggregated Tumors, With High Purity and Good Percentage Recovery: Brief Communication

Hansen, Christopher B.; Gillespie, G. Yancey; Russell, Stephen W.

doi:10.1093/jnci/59.1.273

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Isolation of T-Lymphocytes From Disaggregated Tumors, With High Purity and Good Percentage Recovery: Brief Communication

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Abstract

A combination of two cell separation methods was utilized for the isolation of thymus-derived lymphocytes (TL) from enzymatically disaggregated tumors. Passage through Sephadex G-10-glass bead columns to remove adherent cell types followed by exposure to IgG-coated sheep red blood cell monolayers for removal of Fc receptor-bearing inflammatory cells provided functional TL suspensions of high purity with good percentage recovery.

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Isolation of T-Lymphocytes From Disaggregated Tumors, With High Purity and Good Percentage Recovery: Brief Communication

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Isolation of T-Lymphocytes From Disaggregated Tumors, With High Purity and Good Percentage Recovery: Brief Communication

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