Functional Integrity of Cryopreserved Human Monocytes

Weiner, Roy S.; Normann, Sigurd J.

doi:10.1093/jnci/66.2.255

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Functional Integrity of Cryopreserved Human Monocytes

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Abstract

Human peripheral blood monocytes collected by semicontinuous flow centrifugation were cryopreserved in varying concentrations of dimethyl sulfoxide (DMSO). Optimum protection from lethal cryoinjury was achieved with 7.5–10% DMSO. Compared to fresh cells. cryopreserved cells retained 80% of their chemotactic responsiveness and fructose-6-phosphate shunt activity after stimulation by zymosan. Fresh and cryopreserved human monocytes had comparable but low levels of cytotoxic activity against P-815 murine mastocytoma cells in vitro.

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Functional Integrity of Cryopreserved Human Monocytes

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