The effects of dimethyl sulfoxide (DMSO) on H-2 expression and susceptibility to NK-and cytotoxic T-lymphocyte (CTL)-mediated lysis in the murine T-cell lymphoma YAC-1 and its beta-2-microglobulin (β2m)- deficient variant were studied. Fluorescence-activated cell sorter analysis revealed induction of H-2kk and βm 3 days after culture of YAC-1 with DMSO, where optimal H-2Dd induction required more than 1 week. H-2Kk and H-2Dd induction by DMSO was equal to preteatment of YAC-1 cells with 50-100 and 10-20 U/ml interferon (IFN)-gamma, respectively, but the T-cell differentation antigens Lyt-1, Lyt-2, Thy-1, and L3T4 remained unaffected. DMSO Protected YAC-1 cells from NK lysis as efficently as 10-20 UIFN/ml, whereas susceptibility to anti-H-2a-, H-2Kk-, and H-2Dd-specific CTLs Was augmented as in IFN-treated YAC-1 cells. In contrast, the β2m-deficient variant, which remained H-2 negative at the cell surface after DMSON treatment, also remained NK sensitive. Thus DMSO can induce H-2 expression and alter the sensitivity of murine lymphoma cells to different effector cells. [J Natl Cancer Inst 1988;80:263–269]

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