
Contents
-
-
-
-
-
-
-
-
-
-
-
-
-
-
Introduction Introduction
-
Lysates and Fluids Lysates and Fluids
-
Sample Preparation Sample Preparation
-
Protein Separation Protein Separation
-
In Gel Quantification of Metals In Gel Quantification of Metals
-
Concomitant Metal and Protein Identification Using High-Resolution Mass Spectrometry Concomitant Metal and Protein Identification Using High-Resolution Mass Spectrometry
-
Further Considerations for Ex Vivo Studies Further Considerations for Ex Vivo Studies
-
-
Cells and Tissues Cells and Tissues
-
Imaging Total Metal Imaging Total Metal
-
LA-ICP-MS, Synchrotron μXRF, and Secondary Ion Mass Spectrometry (SIMS) LA-ICP-MS, Synchrotron μXRF, and Secondary Ion Mass Spectrometry (SIMS)
-
Imaging Based on Particle Beam Platforms Imaging Based on Particle Beam Platforms
-
-
Imaging Metal Uptake and Metal Species Imaging Metal Uptake and Metal Species
-
Convergence of Elemental and Molecular Imaging, Possibilities for In Situ Speciation Convergence of Elemental and Molecular Imaging, Possibilities for In Situ Speciation
-
-
-
Living Cells and Animals Living Cells and Animals
-
Fluorescence-Based Sensors That Measure Metal Ions in Cells Fluorescence-Based Sensors That Measure Metal Ions in Cells
-
Sensor Characteristics Sensor Characteristics
-
Dynamic range Dynamic range
-
Factors Biasing Fluorescence Factors Biasing Fluorescence
-
Quantification Quantification
-
Types of Fluorescent Sensors Types of Fluorescent Sensors
-
Current and Future Needs Current and Future Needs
-
-
Tools for Imaging or Assessing Metal–Microbe–Host Interactions in Living Animals Tools for Imaging or Assessing Metal–Microbe–Host Interactions in Living Animals
-
Bioluminescence Imaging Bioluminescence Imaging
-
Fluorescence Imaging Fluorescence Imaging
-
Other Imaging Modalities Other Imaging Modalities
-
Labeled Pathogens Labeled Pathogens
-
Transgenic Reporter Animals Transgenic Reporter Animals
-
-
Tools for In Vivo Imaging and Assessing Metal Uptake, Metal Effects, and Microbes in Humans Tools for In Vivo Imaging and Assessing Metal Uptake, Metal Effects, and Microbes in Humans
-
Single Photon Emission Computed Tomography (SPECT) Single Photon Emission Computed Tomography (SPECT)
-
Positron Emission Tomography (PET) Positron Emission Tomography (PET)
-
Magnetic Resonance Imaging (MRI) Magnetic Resonance Imaging (MRI)
-
Computed Tomography (CT) Computed Tomography (CT)
-
Optical Imaging Optical Imaging
-
Bioassays without Imaging Capacity Bioassays without Imaging Capacity
-
-
Reference Values Reference Values
-
-
Database Development and Relevant Modeling Tools for Predictions about Metals in Biological Systems Database Development and Relevant Modeling Tools for Predictions about Metals in Biological Systems
-
Computational Approaches to Model the Metalloproteome Computational Approaches to Model the Metalloproteome
-
Computational Modeling of Metal Speciation and Homeostasis Computational Modeling of Metal Speciation and Homeostasis
-
Mining Genomic Databases to Identify New Players in Metal Metabolism Linked to the Susceptibility to Infectious Disease Mining Genomic Databases to Identify New Players in Metal Metabolism Linked to the Susceptibility to Infectious Disease
-
-
Future Directions and Challenges Future Directions and Challenges
-
Studying Metals in the Microbe–Host Interaction Studying Metals in the Microbe–Host Interaction
-
Clinical Opportunities, Diagnostics, and the Need for Miniaturization Clinical Opportunities, Diagnostics, and the Need for Miniaturization
-
Additional Considerations for Animal Studies Additional Considerations for Animal Studies
-
-
Summary Summary
-
-
-
-
20 Methods and Technologies for Studying Metals in Biological Systems
Get access-
Published:June 2015
Cite
Abstract
An extensive summary is provided on the methods and methodology available to analyze and image total metals in biological systems as well as to assess their speciation, in particular in metalloproteomes and as free metal ion concentrations. Discussion focuses on instrumental methods for analysis and separation and how they are complemented by genetic and bioinformatics approaches. The treatment of methods follows increased complexity and experimental challenges: from lysates and fluids, to cells and tissues, to living cells and animals, and finally to the prospects of applying extent technology to investigations in humans. Presently, method sensitivity is not sufficient for analysis of metals in the pathogen in vivo. Future analytical needs are presented to address metal ions in host–pathogen interactions for diagnosis and treatment of infectious disease. The material is presented in the context of both redistribution of metals in the host and known mechanisms of warfare to acquire the transition metal ions that are essential for growth and survival of either host or pathogen.
Sign in
Personal account
- Sign in with email/username & password
- Get email alerts
- Save searches
- Purchase content
- Activate your purchase/trial code
- Add your ORCID iD
Purchase
Our books are available by subscription or purchase to libraries and institutions.
Purchasing informationMonth: | Total Views: |
---|---|
August 2023 | 1 |
August 2024 | 1 |
Get help with access
Institutional access
Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:
IP based access
Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.
Sign in through your institution
Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.
If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.
Sign in with a library card
Enter your library card number to sign in. If you cannot sign in, please contact your librarian.
Society Members
Society member access to a journal is achieved in one of the following ways:
Sign in through society site
Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:
If you do not have a society account or have forgotten your username or password, please contact your society.
Sign in using a personal account
Some societies use Oxford Academic personal accounts to provide access to their members. See below.
Personal account
A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.
Some societies use Oxford Academic personal accounts to provide access to their members.
Viewing your signed in accounts
Click the account icon in the top right to:
Signed in but can't access content
Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.
Institutional account management
For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.