Impact of alanyl-tRNA synthetase editing deficiency in yeast

Abstract Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes that provide the ribosome with aminoacyl-tRNA substrates for protein synthesis. Mutations in aaRSs lead to various neurological disorders in humans. Many aaRSs utilize editing to prevent error propagation during translation. Editing defects in alanyl-tRNA synthetase (AlaRS) cause neurodegeneration and cardioproteinopathy in mice and are associated with microcephaly in human patients. The cellular impact of AlaRS editing deficiency in eukaryotes remains unclear. Here we use yeast as a model organism to systematically investigate the physiological role of AlaRS editing. Our RNA sequencing and quantitative proteomics results reveal that AlaRS editing defects surprisingly activate the general amino acid control pathway and attenuate the heatshock response. We have confirmed these results with reporter and growth assays. In addition, AlaRS editing defects downregulate carbon metabolism and attenuate protein synthesis. Supplying yeast cells with extra carbon source partially rescues the heat sensitivity caused by AlaRS editing deficiency. These findings are in stark contrast with the cellular effects caused by editing deficiency in other aaRSs. Our study therefore highlights the idiosyncratic role of AlaRS editing compared with other aaRSs and provides a model for the physiological impact caused by the lack of AlaRS editing.

The editing function of aaRSs is mostly conserved from bacteria to higher eukaryotes throughout evolution. It is therefore puzzling that disrupting editing of some aaRSs appears to be well tolerated in bacteria and eukaryotes. For instance, ablating the editing function of threonyl-tRNA synthetase (ThrRS) has little effect on the growth of Escherichia coli (17,18). Editing deficiency in phenylalanyl-tRNA synthetase (PheRS) also shows no growth effect in E. coli unless an oxidized form of tyrosine (meta-tyrosine) accumulates (19). In yeast, leucyl-tRNA synthetase (LeuRS) editing defects does not affect growth under normal conditions (20). In contrast, mild editing defects in alanyl-tRNA synthetase (AlaRS) lead to neurodegeneration (loss of Purkinje cells) and cardiac proteinopathy in mice, and a more severe AlaRS editing defect is embryonic lethal (21,22). In humans, an AlaRS mutation that causes both aminoacylation and editing defects has been associated with patients suffering early-childhood neurodegeneration and progressive microcephaly (23). AlaRS editing deficiency appears to selectively damage neuron cells and cardiomyocytes. Although elevated levels of protein ubiquitination and aggregation have been observed in these cells of the editing-defective mice (21,22), the overall cellular impact of AlaRS editing remains elusive. In this study, we use Saccharomyces cerevisiae as a model organism to compare the cellular impact of AlaRS editing and aminoacylation defects, respectively. Combining RNA sequencing, proteomics, genetic, and biochemical analyses, we show that editing deficiency in AlaRS activate the general amino acid control (GAAC) pathway and causes severe growth defects under heat stress. We further show that the heat sensitivity caused by AlaRS editing deficiency is partially due to impaired carbon metabolism, which may lead to a weakened heatshock response.

Plasmids and strains
All S. cerevisiae strains used here were derivatives of BY4741. Escherichia coli DH5␣ grown in LB medium were used for cloning. ALA1 (encoding AlaRS) point mutants C719A and G906D were obtained by plasmid shuffling. Briefly, pRS315-ALA1-C719A and pRS315-ALA1-G906D were transformed into BY4741 ala1 carrying pRS316-ALA1-WT using the lithium acetate method, and the URA3 pRS316 plasmid was cured by counter selection on 5fluoroorotic acid containing synthetic defined (SD)-Leu agar plates.
To create the gcn2 , gcn4 , alaX , ltn1 , rqc1 , rqc2 , eap1 and caf20 mutants, the coding regions were replaced with URA3 or HIS2 gene. Cells were grown in either YPD (1% yeast extract, 2% peptone and 2% glucose) media or SD dropout medium (0.17% yeast nitrogen base, 0.5% ammonium sulfate, 2% glucose and 0.14% amino acid dropout mix without His, Leu or Ura). All strains and plasmids are listed in Supplementary Table S4 and all the  primers used are listed in Supplementary Table S5.
The substrate of editing assays [ 14 C] Ser-tRNA Ala was prepared by charging 20 mg/ml total yeast tRNAs with 100 M [ 14 C] Ser (67 cpm/pmol) by the editing-defective human AlaRS 723A mutant in aminoacylation buffer at 37 • C for 10 min. 1 M yeast AlaRS was then added to hydrolyze Ser-tRNA. At each time point, an aliquot was spotted on 3MM Whatman paper discs presoaked with 5% TCA, washed three times with 5% TCA, dried, and scintillation counted.

Temperature sensitivity analysis
Liquid culture growth assays were tested using Synergy HT microplate reader (BioTek, USA) at 30 or 37 • C. WT, C719A, G906D and their gcn2 , gcn4 and alaX derivative strains were grown in YPD, and tRNA overexpression strains were grown in SD-Leu. For agar plating assay, cells were grown to log phase in liquid media at 30 • C. 5-or 10fold serial dilutions were spotted on YPD or SD -Leu agar plates and incubated both at 30 • C and 37 • C for 2-4 days before imaging.

␤-Lactamase assay
To determine Ser misincorporation at Ala codons, WT, C719A, G906D cells expressing the WT (pJL-bla) or S68A mutant (pJL-bla-S68A-GCC) ␤-lactamase or carrying the control plasmid pJD1212 were grown in SD-Ura to log phase. The same OD 600 (0.9) of cells were collected, washed and resuspended in 200 l lysis buffer (PBS pH 7.0, 1 mM phenylmethylsulfonyl fluoride (PMSF), 10 mM dithiothreitol (DTT), 100× protein inhibitor cocktail and 3 mg/ml zymolyase) and incubated at room temperature for 40 min. ␤-Lactamase activity (25) was determined using a 100 l reaction mixture containing PBS pH7.0, 100 M nitrocefin and 10 l cell lysate. ␤-Lactamase with Ser at position 68 converts nitrocefin to a product with absorption at 486 nm, which was measured using the Synergy HT microplate reader.

␤-Galactosidase assay
To determine the translational regulation of GCN4, WT, C719A or G906D yeast cells with Pgcn4-lacZ reporters pJD821, pJD822 and pJD823 were grown in SD-Ura medium to log phase at 30 • C. 700 l culture were collected and resuspended in 700 l Z-buffer (60 mM Na 2 HPO 4 , 40 mM NaH 2 PO 4 , 10 mM KCl and 1 mM MgSO 4 ), and cell density was measured by OD 600 . Cells were lysed by adding 100 l chloroform and 50 l 0.1% SDS and vortexing for 15 s. Reaction was initiated by addition of 0.2 ml pre-warmed ONPG (o-nitrophenyl-␤-galactoside, 4 mg/ml in Z-buffer); after yellow color developed, the reaction was terminated by adding 500 l 1 M Na 2 CO 3 . Cell debris was removed by centrifugation, and OD 420 of the supernatant was determined. ␤-Galactosidase activity was calculated according to the following equation: Miller units = 1000 × OD 420 /(T × V × OD 600 ) (26,27).

Isolation of aggregated proteins
Aggregated proteins were prepared as previously described (28). Briefly, 1 ml log-phase yeast cells were washed and harvested, and the pellets were resuspended in 200 l lysis buffer (PBS pH7.0, 10 mM DTT, 1 mM PMSF, 100× protease inhibitor cocktail, and 3 mg/ml zymolyase), then incubated at room temperature for 40 min with 20 min intervals for vortexing. After brief sonication, the samples were centrifuged for 20 min at 200 g at 4 • C. Supernatants were then adjusted to the same protein concentration using Bradford method, and the aggregates were collected by centrifuging at 16 000 g for 20 min. After removal of the supernatant, aggregated proteins were washed twice with 2% NP-40 in PBS, sonicated and centrifuged at 16 000 g for 20 min. The aggregated proteins were then washed twice with PBS and sonicated. The aggregates and equal amounts of total proteins were then boiled in SDS loading buffer and separated on 12% SDS-PAGE gels, and finally analyzed by Coomassie brilliant blue staining.

Determination of heatshock response activity
The live-cell luciferase assay was performed essentially as described (29). WT, C719A and G906D strains containing the heatshock response reporter pHSE-lucCP + and the control salt inducible reporter pGre2-lucCP + were grown in SD-His at 30 • C to log phase and adjusted to the same concentration. Prior to checking the heat-induced luminescence, pGre2-lucCP + harboring strains were incubated with 0.4 M NaCl, and all strains were shaken at 30 • C for 1 h. 500 M luciferin was added to all strains in a 96-well plate, which was immediately placed in a 37 • C microplate reader to record the luminescence intensity.
The heatshock response reporter pHSE-GFP was transformed into the WT, C719A, G906D strains and the resulting transformants were grown in SD-Ura medium to log phase. To examine heat-induced GFP expression, strains were adjusted to the same OD 600 and placed in a 37 • C microplate reader for fluorescence measurement.

Fluorescence microscopy
The heat-induced aggregation reporter pHSP104-RFP (30) was transformed into the WT, C719A and G906D strains, and the resulting transformants were grown in SD-Ura medium to log phase. 1 l yeast cells were pipetted onto a 2 l 1% agarose PBS pad on a 15-well slide. Cells were visualized on a BZ-X810 inverted microscope (Keyence).

Ribosome quality control analysis
Ribosome quality control reporter was tested as described (31). The WT, C719A, and G906D strains and their ltn1 , rqc1 and rqc2 strains carrying the pTDH3-GFP-R12-RFP were grown in SD-Ura. Cell pellets were resuspended in 200 l lysis buffer (PBS pH7.0, 10 mM DTT, 1 mM PMSF, 100× protease inhibitor cocktail, and 3 mg/ml zymolyase), and incubated at room temperature for 30 min with 15 min intervals for vortexing. After sonication and centrifugation (16 000 g for 5 min), supernatant proteins were separated by 12% SDS-PAGE and transferred to nitrocellulose membranes. Next, standard western blotting procedure was followed to detect the GFP levels. The mouse anti-GFP first antibody (Invitrogen) with 1:1000 dilution and goat anti-mouse IgG-HRP secondary antibody (Invitrogen) with 1:5000 dilution were used. Nitrocellulose membranes were treated with ECL chemiluminescent substrate reagents (Bio-Rad) and visualized using a ChemiDoc Imaging System (Bio-Rad).

Protein synthesis assay
To quantify the global protein synthesis rate, log-phase WT, C719A and G906D cells were adjusted to the same OD 600 , washed twice with SD-methionine (Met) medium, and then the protocol of Click-iT ® HPG Alexa Fluor ® 594 protein synthesis assay kit (Thermo Fisher) was followed. This method is based on the co-translational incorporation of HPG (L-homopropargylglycine), which is an analog of methionine containing an alkyne moiety. Cell pellets were incubated with 100 l SD -Met containing 50 M HPG at 30 or 37 • C for 1 h. Following fixation and fluorophore labeling, the A 600 and the fluorescence signal intensity were determined using a Synergy HT microplate reader.

Transcriptome analysis
WT, C719A, and G906D cells were grown in YPD at 30 • C to log phase, and incubated at 37 • C for additional 0, 1 or 2 h. Total RNA was prepared using an RNA extraction kit (Qiagen). Library construction and Illumina sequencing were performed by Novogene.

Label-free quantitative proteomics analysis
Label-free quantitative proteomics was performed by Creative Proteomics (New York, USA). Three biological replicate samples of each strain were prepared and analyzed. Log-phase WT, C719A and G906D cells were incubated at 30 or 37 • C for 2 h. Cell pellets from 2 ml cultures were fast frozen in liquid nitrogen. Protein extraction, trypsin digestion, and LC-MS/MS analysis were performed by Creative Proteomics (New York, USA). The resulting MS/MS data were processed using MaxQuant software. Gene expression with 1.5-fold difference of label-free quantitation (LFQ) intensity and P value <0.05 was considered significant. The identified differentially expressed proteins were enriched by Gene Ontology (GO) annotation and KEGG pathway analyses using metascape (32).

AlaRS editing deficiency causes serine misincorporation and heat sensitivity
Alanyl-tRNA synthetase misacylates serine (Ser) and glycine onto tRNA Ala and uses both a cis-editing site and trans-editing factors to hydrolyze the misacylated tR-NAs (14,(34)(35)(36)(37). To evaluate the cellular effects of editing and aminoacylation defects, we constructed haploid yeast strains carrying the wild type (WT), C719A or G906D AlaRS variants on a single-copy plasmid in the AlaRS (ALA1) deletion background. The C719A mutation in yeast AlaRS is equivalent to C666A in E. coli and C723A in humans that selectively inactivate editing but not aminoacylation (18,23,34) Figure S1). The G906D mutation in yeast is equivalent to G913D in humans, which is identified in microcephaly patients and decreases the aminoacylation but not editing efficiency (23). Our in vitro experiments using recombinant yeast AlaRS variants also confirm that C719A and G906D mutations cause editing and aminoacylation defects, respectively (Supplementary Figure S2). We additionally attempted to construct a mutant strain carrying a truncated AlaRS identified in microcephaly patients with both aminoacylation and editing defects (23) but were unsuccessful, suggesting that this mutant AlaRS is not able to support yeast growth.
To evaluate mistranslation rates resulting from AlaRS editing deficiency, we transformed a ␤-lactamase gene with a critical active-site Ser (S68) codon changed to an alanine (Ala) codon and quantitated the enzyme activity in yeast. Misincorporation of Ser into the A68 position would yield active ␤-lactamase proteins (16,18,25). The C719A mutation increased Ser misincorporation 7-fold from 0.03% to 0.2%, whereas the G906D mutation did not affect Ser misincorporation ( Figure 1A). Additional Ser in the media further increased the Ser misincorporation rate to 0.4% in the C719A strain. These results confirm that the C719A mutation causes an AlaRS editing defect in yeast and promotes mistranslation despite the presence of the trans-editing factor AlaX.
We next tested growth of the resulting strains at various temperatures. Both the C719A and G906D mutant strains grew as well as the WT at 30 • C, but the C719A strain displayed severe growth defects both on the yeast peptone dextrose (YPD) agar plate and in YPD liquid culture at 37 • C, suggesting that AlaRS editing deficiency leads to increased sensitivity to heat ( Figure 1B, C and Supplementary Figure S3). To test whether such heat sensitivity is due to Ser mistranslation, we expressed tRNA Ser mutants with Ala anticodons (AGC and TGC) in WT yeast. Seryl-tRNA syn-thetase does not recognize the tRNA anticodon and therefore aminoacylates such tRNA variants with Ser, resulting in misreading of Ala codons as Ser. We found that similar to the AlaRS C719A mutation, tRNA Ser AGC and tRNA Ser TGC both caused severe growth defects at 37 • C ( Figure 2). In contrast, misincorporation of Ser at leucine (Leu) codons by tRNA Ser CAG did not appear to cause heat sensitivity (Supplementary Figure S4). These results suggest that Ser misincorporation at Ala codons is particularly toxic under heat stress conditions.

Transcriptomics and proteomics analyses of AlaRS mutants
To gain a better understanding of how AlaRS editing and aminoacylation defects influence gene expression in yeast, we performed RNA sequencing analyses of the WT, C719A and G906D strains that were pre-grown at 30 • C, before and after shifting to 37 • C heat stress (Figure 2, Supplementary Figures S5 and S6, Table S1). Compared with the WT, both C719A and G906D strains showed significant upregulation in the ribosome and amino acid biogenesis pathways at 30 or 37 • C. The most significantly downregulated pathways caused by the C719A mutation upon heat stress were carbon metabolism and the citrate cycle ( Figure 3).
While RNA sequencing analysis is a powerful tool to profile the transcriptome and understand regulation of gene expression, the mRNA levels may not always correlate with the protein levels due to translational and posttranslational regulations (38). We therefore performed additional labelfree quantitative proteomics experiments. We found that like the RNA sequencing results, proteomics results revealed that upon heat stress ribosome and amino acid biogenesis were significantly upregulated in the C719A strain, and carbon metabolism was most significantly downregulated ( Figure 4, Supplementary Tables S2 and S3). Interestingly, the protein folding pathway that include many heatshock proteins was also downregulated in the C719A strain. The proteome of the G906D strain was less consistent with the transcriptome (Supplementary Figure S7, Supplementary Tables S2 and S3). In particular, the amino acid biosynthesis proteins were less abundant in the G906D mutant compared with the WT, which was opposite to the mRNA levels, implicating additional translational and posttranslational control of this pathway.

AlaRS editing deficiency activates the general amino acid control pathway
Our RNA sequencing and proteomics data both suggest that the C719A mutation causing AlaRS editing deficiency activates the amino acid biosynthesis pathway. In yeast, amino acid biosynthesis is controlled by the Gcn4p transcription factor, which regulates the GAAC pathway (39)(40)(41)(42). Upon amino acid starvation, uncharged tRNAs accumulate and activate Gcn2p to phosphorylate eIF2A, which attenuates overall protein synthesis but selectively induces translation of GCN4 via bypassing the open reading frame uORF4 ( Figure 5A). GAAC thus provides a feedback mechanism to maintain amino acid homeostasis. To test the GAAC response, we used a lacZ reporter fused with the 5 region (with or without uORFs) of GCN4. The plasmid pJD821 contains all the regulatory elements of GCN4 translation, whereas pJD822 and pJD823 serve as constitutively repressed and derepressed controls, respectively (Figure 5A). We confirmed the RNA sequencing data that the GAAC pathway was indeed activated in the C719A and G906D strains compared with the WT (Figure 5B and Supplementary Figure S3C). To test whether GAAC was involved in the heat sensitivity of the editing-defective strain, we deleted the GCN4 and GCN2 genes separately in the WT, C719A and G906D strains. Neither GCN4 or GCN2 deletion rescued growth of the C719A strain at 37 • C ( Figure  5C and D), suggesting that the heat sensitivity phenotype caused by AlaRS editing deficiency is independent of the GAAC pathway.

AlaRS editing deficiency attenuates translation
Phosphorylation of eIF2A by activated Gcn2p attenuates global translation initiation (39). We therefore measured the overall protein synthesis rate using a methionine analog homopropargylglycine (HPG). We found that HPG incorporation in the C719A strain was decreased over 50% at both 30 and 37 • C compared with the WT, and the G906D mutant also had a lower translation efficiency at 37 • C ( Figure  6). Deleting GCN2 did not restore protein synthesis in the AlaRS mutant strains. In yeast, there are two characterized binding proteins (Eap1p and Caf20p) that inhibit eIF4E during translation initiation (42), prompting us to test the role of Eap1p and Caf20p in repression of translation by the C719A AlaRS mutation. Deleting CAF20, but not EAP1, partially restored HPG incorporation in the C719A mutant (Supplementary Figure S8A and S8B). However, neither Caf20nor Eap1fully rescued heat sensitivity of the C719A strain (Supplementary Figure S8C).

AlaRS editing deficiency dampens the heatshock response
The proteomics data revealed that the abundance of heatshock proteins was decreased in the proteome of the AlaRS C719A strain (Figure 4), prompting us to investigate the heatshock response in the editing and aminoacylation defective strains. Using both luciferase and green fluorescence protein (GFP) reporters under the control of the heatshock element (HSE) promoter (29,43), we found that the heatshock response was significantly reduced in the C719A strain compared with the WT (Figure 7). The heatshock response activates expression of chaperones to protect cells from protein denaturing conditions such as heat (44,45). We further tested protein aggregation by purifying aggregates and electrophoresis on SDS-PAGE gel, as well as using a HSP104-RFP (red fluorescence protein) reporter (30). The C719A mutation did not appear to substantially increase total protein aggregates or the number of aggregated foci (Supplementary Figure S9). In yeast, a ribosomal quality control (RQC) mechanism adds Ala and Thr tails to stalled peptides and target them for degradation (31). The RQC process requires LTN1, RQC1 and RQC2, and contributes to protein quality control and heat resistance (31,43). Using a reporter for the RQC pathway (31), we showed that there was no difference in RQC among WT, C719A or G906D strains (Supplementary Figure S10), ruling out the role of RQC in our observed heat sensitivity of the AlaRS editingdefective strain.

Extra carbon source partially rescues heat sensitivity caused by AlaRS editing deficiency
The most significantly downregulated pathway in the C719A strain, as revealed by both RNA sequencing and proteomics analyses, is carbon metabolism (Figures 3 and  4). Impaired carbon metabolism is expected to decrease synthesis of amino acids. In support of this notion, we found that supplementing the glucose media with extra glycerol carbon source suppressed the GCN4 GAAC response in the C719A strain (Supplementary Figure S11). Interestingly, additional carbon sources also partially rescued the growth defect of C719A at 37 • C (Figure 8), likely through increasing heatshock proteins and energy source to properly fold mistranslated proteins.

DISCUSSION
Decades of biochemical and structural studies have elucidated the mechanisms of editing by aminoacyl-tRNA synthetases and trans-editing factors (9,10,12,14,24,(35)(36)(37)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56). However, why editing is conserved throughout evolution in many aaRSs has been a perplexing question given that it is dispensable under normal growth conditions. Based on our systematic analyses in yeast, we propose the following model for the function of AlaRS editing ( Figure  9): AlaRS uses a cis-editing domain to prevent accumulation of misacylated tRNA Ala , such as Ser-tRNA Ala ; AlaRS editing deficiency causes misincorporation of Ser into Ala positions in the proteome, which leads to a cascade of misfolding and degradation events of key regulatory proteins; Heat stress exacerbates protein misfolding and results in loss of function for key factors in the carbon metabolism, heatshock response and protein synthesis pathways. Exactly which key regulatory proteins are affected by Ser misincorporation remain to be elucidated in future studies. As a result of impaired carbon metabolism and heatshock response, the GAAC pathway is activated to compensate for the amino acid biosynthesis deficiency, and the cells also become sensitive to heat stress. This is supported by our observations that extra carbon source suppresses the GAAC response and partially rescues the heat sensitivity of the    editing-defective strain (Figure 8 and Supplementary Figure S8). The GAAC pathway is known as the integrated stress response (ISR) in mammals (57,58). A previous study reveals that a mutation in tyrosyl-tRNA synthetase (TyrRS) that leads to peripheral neuropathy activate ISR by localizing TyrRS in the nucleus (59). In future studies, it would be important to systematically characterize the role of diseaseassociated aaRS mutations in regulation of ISR, as well as the underlying mechanisms.
Editing deficiency of many aaRSs are well tolerated under normal growth conditions. Valyl-and phenylalanyl-tRNA synthetase editing-defective E. coli mutants grow as well as the WT unless their editing substrates accumulate in large amounts in the cells (19,60). ThrRS editing deficiency causes growth inhibition only when the heatshock proteases are absent (25). Recent studies suggest that aaRS editing could play critical roles under stressed conditions. For instance, lack of PheRS editing tempers the GAAC pathway in yeast due to accumulation of misacylated tRNA Phe (61).
Interestingly, AlaRS editing deficiency shows the opposite effect as PheRS and constitutively activates the GAAC response (Figures 3-5). We also show that ablating AlaRS editing attenuates the heatshock response and causes heat sensitivity (Figures 1, 4 and 7). In contrast, LeuRS editing deficiency as well as Ala misincorporation at Pro codons activate the heatshock response (20,62). It becomes increasingly clear that the editing function of different aaRSs may regulate distinct cellular response pathways. This is likely due to the nature of amino acid misincorporation resulting from different editing defects. Ser misincorporation at Ala codons due to AlaRS editing deficiency should exhibit distinct effects on the proteome as misincorporation at Leu codons. In support of this notion, whereas the tRNA Ser variants (AGC and TGC) decoding Ala codons cause heat sensitivity, the tRNA Ser variant (CAG) decoding Leu does not (Figure 2 and Supplementary Figure S4). Indeed, CTG Leu codons are ambiguously decoded as both Leu and Ser in Candida albicans, which promotes phenotypic diversity (63,64).
AlaRS is a unique aaRS in several aspects. While most aaRSs recognize the anticodons of tRNAs, AlaRS recognizes a single G3:U70 pair as the major tRNA determinants for both aminoacylation and editing (65)(66)(67)(68). Editing defects in AlaRS also appear to be particularly detri-mental, as evidenced by the severe neurological and cardiovascular disorders resulting from editing-defective AlaRS mutants in mice (21,22). Intriguingly, an AlaRS mutation (p.Tyr690Leufs*3) that lead to both aminoacylation and editing defects are found in human patients with progressive microcephaly and epileptic encephalopathy (23). The other AlaRS allele in these patients carry a mutation (G913D, equivalent to G906D in yeast) that decreases the aminoacylation efficiency. It is unclear how AlaRS editing deficiency contributes to the disease onset in the patients. We attempted to introduce both AlaRS mutations in yeast but were only able to obtain the G906D mutant strain, Figure 9. Model of cellular impact caused by AlaRS editing deficiency. AlaRS harbors catalytic (aminoacylation) and editing domains. Editing deficiency in AlaRS leads to accumulation of Ser-tRNA Ala and Ser misincorporation at Ala codons. Ser mistranslation promotes misfolding, degradation and loss of function for some key regulatory proteins, resulting in defects in translation, heatshock response and carbon metabolism.
suggesting that a combination of severe aminoacylation and editing defects in AlaRS is lethal for yeast. This could be explained by loss of function resulting from AlaRS editing deficiency, which may destabilize the proteome and dysregulate key cellular pathways.

CONCLUDING REMARKS
In summary, our work here reveals the critical function of AlaRS editing in maintaining normal metabolism and stress responses in yeast cells, which is distinct from other aaRSs. The physiological roles of translational fidelity may vary due to the different nature and severity of translational errors.
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE (33) partner repository with the dataset identifier PXD026930.