Abstract

The PDC1 gene of Saccharomycescerevisiae, encoding pyruvate decartoxylase was sequenced. The gene contains an cen reaiing frame of 1647 base pairs. The codon usage shows the same strong bias as fcur1 for some other glycolytic enzymes. Transcription starts mainly at −30 and terminates 100 base pairs downstream of the termination codon. In some strains a second termination site, 46 base pairs upatream of the stcp codon was oheerved.

The function of the promoter region was analyzed by fusion to the bacterial structural gene encoding ℓ-lactamase (bla. C multicopy plasmid or integrated in the genome, the expression of the bla gene showed the regulation of the authentic PDC1 gene.

Comments

0 Comments