Telomere shortening in human HL60 cells by treatment with deoxyguanosine analogs

Abstract

Telomerase is a cellular endogenous reverse transcriptase thought to play an important role in the maintenance of telomere length. We investigated the effects of 3′-azido-2′,3′-dideoxyguanosine (AZddG) and carbocyclic oxetanocin G (C.OXT-G), of which the triphosphate derivatives AZddGTP and C.OXT-GTP show potent telomerase inhibition, on telomere length of human HL60 cells in culture. Although AZddG caused more significant telomere shortening than C.OXT-G, only a slight decrease of cell growth rate was observed.

Author notes

1Department of Biological Sciences, Teikyo University of Science and Technology, Uenohara, Yamanashi 409-0193, Japan, 2Biotechnology Research Center, Teikyo University of Science and Technology, Uenohara, Yamanashi 409-0193, Japan, 3Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, Shido, Sanuki City, Kagawa 769-2193, Japan