In South Andes lakes, zooplankton succession is characterized by a change in the dominance from the calanoid copepod Boeckellagracilipes in winter and spring, to the cladoceran Bosminalongirostris in midsummer, and the rotifer Polyarthravulgaris in late summer. We performed three series of field experiments at different times (late spring, summer and late summer) to examine the role of zooplankton constitution in the released nitrogen:phosphorus (N:P) ratio. We observed that changes in the zooplankton constitution over the annual cycle may change the nutrient supply ratio. In South Andes lakes, Boeckella gracilipes would decrease the P limitation, lowering the N:P ratio, whereas Bosmina longirostris tends to increase the N:P ratio and therefore increase the P limitation during summer.