https://orcid.org/0009-0003-5868-381X
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Julia Legen, Benjamin Lenzen, Nitin Kachariya, Stephanie Feltgen, Yang Gao, Simon Mergenthal, Willi Weber, Enrico Klotzsch, Reimo Zoschke, Michael Sattler, Christian Schmitz-Linneweber, A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis, The Plant Cell, Volume 36, Issue 8, August 2024, Pages 2851–2872, https://doi.org/10.1093/plcell/koae145
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Abstract
Arabidopsis (Arabidopsis thaliana) plants can produce photosynthetic tissue with active chloroplasts at temperatures as low as 4°C, and this process depends on the presence of the nuclear-encoded, chloroplast-localized RNA-binding protein CP29A. In this study, we demonstrate that CP29A undergoes phase separation in vitro and in vivo in a temperature-dependent manner, which is mediated by a prion-like domain (PLD) located between the two RNA recognition motif domains of CP29A. The resulting droplets display liquid-like properties and are found near chloroplast nucleoids. The PLD is required to support chloroplast RNA splicing and translation in cold-treated tissue. Together, our findings suggest that plant chloroplast gene expression is compartmentalized by inducible condensation of CP29A at low temperatures, a mechanism that could play a crucial role in plant cold resistance.
