The kinesin motor POS3 and the microtubule polymerase MOR1 coordinate chromosome congression during mitosis in Arabidopsis

Abstract

Faithful chromosome segregation during mitosis is crucial for eukaryotic organisms. Centromere-associated protein-E (CENP-E), a kinetochore-localized kinesin motor, facilitates chromosome congression during mitosis in animals. However, it remains unclear whether plants rely on kinesins similar to CENP-E for chromosome alignment. In our genetic screens for Arabidopsis (Arabidopsis thaliana) mutants that are hypersensitive to the microtubule-destabilizing drug propyzamide, we identified propyzamide oversensitive3-1 (pos3-1), which harbors a mutation in a kinesin-like protein that shares sequence similarity with the N-terminal region of CENP-E. We demonstrated that POS3 dynamically associates with kinetochores during chromosome congression and segregation in mitosis. Moreover, loss of POS3 results in prolonged mitosis, increased aneuploidy, and misaligned chromosomes near the spindle poles. Unexpectedly, we discovered a direct physical interaction and functional link between POS3 and the microtubule polymerase MICROTUBULE ORGANIZATION1 (MOR1) in regulating chromosome alignment and segregation during mitosis. Finally, we showed that MOR1 is required for the kinetochore localization of POS3 in mitosis. Together, our findings establish the vital role of POS3 in chromosome congression and uncover a functional link between POS3 and MOR1 that is essential for proper chromosome alignment and segregation in plant mitosis.