Chemical screening approach using single leaves identifies compounds that affect cold signaling in Arabidopsis

A chemical screening approach using single leaves identifies 1,4-naphthoquinones as compounds that affect cold signaling in Arabidopsis.


Introduction
Adaptation and responses to abiotic stresses, such as low temperature and drought, are indispensable for land plants to sustain life.When plants are exposed to such stresses, they remodel their transcriptome to adapt to their environment (Uemura et al. 1995;Thomashow 2001;Zhu 2001).These processes are primarily mediated by transcription factors and upstream signaling pathways.However, we are currently far behind a complete understanding of the signaling pathways involved.One good example is the response to low-temperature stress.Although it is well known that lowtemperature signaling is mediated by C-repeat binding factor/dehydration-responsive element-binding (CBF/DREB) transcription factors (Shinozaki et al. 2003), low temperature sensors remain to be identified in Arabidopsis (Arabidopsis thaliana) (Guo et al. 2018).Therefore, the development of methodologies is critical for further elucidating the signaling pathways in plants.
The identification of chemical compounds that affect intracellular signaling and trafficking has greatly contributed to our understanding of developmental regulation in plants (Hicks and Raikhel 2014;Nakamichi et al. 2022).In most cases, these compounds have been identified using germinating seedlings (∼1-wk-old seedlings at best) or cell cultures (Armstrong et al. 2004;Zhang et al. 2012;Kailasam et al. 2018;Jay et al. 2019;Uehara et al. 2019;Shirakawa et al. 2021).
Chemical screening is performed at this stage using a 96-well plate.However, it is challenging to adopt the same strategy for mature plants because of their body size.Since the lifetime of Arabidopsis is longer than 2 months, performing chemical screening using mature plant tissues would be of great advantage.The establishment of such a screening system would allow us to better understand the signaling pathways that regulate plant growth and development during the mature stage.
In the present study, we established a high-throughput screening method using single leaves of mature plants to identify small molecules that affect cold-regulated gene expression.We used transgenic Arabidopsis harboring the COLD-REGULATED 15A (COR15A) promoter::luciferase (COR15Apro::LUC) construct as a model, because it allows the monitoring of gene expression in a nondestructive manner.A single leaf excised from COR15Apro::LUC plants exhibited both cold-regulated and abscisic acid (ABA)-dependent induction of luciferase activity in a microwell plate.Using this system, we screened a library of ∼500 natural compounds to identify molecules that affect the cold induction of COR15Apro::LUC.Among the compounds identified, we found that derivatives of 1,4-naphthoquinone specifically inhibit the cold induction of COR15Apro::LUC.These molecules appeared to inhibit the rapid induction of upstream CBF transcription factors upon exposure to low temperature, suggesting that 1,4-naphthoquinones alter upstream signaling processes.Our study offers a chemical screening scheme for identifying compounds that adjust the environmental responses of developed plants.This type of analysis is likely to reveal an unprecedented link between certain compounds and plant environmental responses.

The excised leaves of COR15Apro:LUC plants grown in submerged culture can be used to achieve high-throughput chemical screening
To achieve high-throughput screening of chemical compounds in mature tissues, we first generated transgenic Arabidopsis carrying a luciferase reporter gene.As a model system, we selected the promoter region of COR15A, a wellcharacterized gene that is strongly induced by lowtemperature treatment (Lin and Thomashow 1992;Baker et al. 1994;Nakayama et al. 2007).The 5′-upstream region of COR15A was fused to firefly luciferase to create the COR15Apro::LUC construct (Fig. 1A).We grew transgenic Arabidopsis carrying COR15Apro::LUC in submerged cultures (Fig. 1B, left) because this growth condition causes hyperhydricity of true leaves without substantial developmental effects (Ohyama et al. 2008).As visualized by a chargecoupled device (CCD) camera, COR15Apro::LUC plants responded to low temperatures (Fig. 1C).Furthermore, we confirmed that those plants could be cold acclimated, as they showed increased freezing tolerance (Supplemental Fig. S1).
We next examined whether excised leaves could respond similarly to low temperatures.Single leaves of COR15Apro:: LUC plants grown in submerged cultures were excised and placed into 96-well microplates containing liquid Murashige-Skoog (MS) medium (Figs. 1B,right,and S2).The leaves were then exposed to low temperature for 48 h.As shown in Fig. 1D, the luciferase activity of cold-treated leaves was ∼100 times higher than that of nontreated leaves.We also investigated the effects of leaf size and age on cold induction of luciferase activity.Leaves that exhibited differences in size and age were selected from multiple COR15Apro::LUC plants grown in submerged cultures (Supplemental Fig. S2).Measurement of cold-induced luciferase activity indicated that differences in size and age did not affect low-temperature responses (Supplemental Figs.S2 and S3), confirming the stability of assay system.Although we decided to use a microplate reader for our subsequent assay, we found that a CCD camera was also applicable for the assay (Supplemental Fig. S4).We also confirmed that endogenous COR15A was induced under the same conditions (Fig. 1E), indicating that luciferase activity in excised COR15Apro::LUC leaves can be used to monitor lowtemperature responses.
To further confirm that luciferase activity in excised COR15Apro::LUC leaves could be used for chemical screening, we treated the leaves with ABA and cycloheximide (CHX).ABA treatment induces COR15A expression in Arabidopsis plants (Baker et al. 1994), and CHX inhibits eukaryotic protein synthesis and has been shown to inhibit cold acclimation in multiple plant species (Ennis and Lubin 1964;Chen et al. 1983;Gilmour et al. 1988).Treatment of nonacclimated leaves with ABA for 4 h strongly induced luciferase activity (Fig. 1D).In contrast, when leaves were treated with CHX during lowtemperature treatment, their luciferase activity after lowtemperature exposure was only ∼1% that of control leaves (Fig. 1D).
These data indicate that luciferase activity in the excised leaves of COR15Apro::LUC plants grown in submerged cultures can be used for chemical screening.Furthermore, ABA and CHX can serve as positive controls for activation and inhibition in chemical screening, respectively.

Screening of natural compounds that affect cold-regulated induction of COR15Apro::LUC
We screened 502 compounds from a natural product library (Enzo Life Sciences; Supplemental Table S1) using excised leaves from COR15Apro::LUC plants.Luciferase activity was measured using a Varioskan Flash microplate reader, which determined luciferase activity per leaf directly (Supplemental Fig. S2C).
Based on the average of at least 2 independent leaves, we first searched for compounds that promoted luciferase activity without cold acclimation (Fig. 2A).ABA at a final concentration of 100 μM and DMSO were used as positive and negative controls, respectively, and luciferase activity in ABA-treated leaves was set to 100%.Then, we screened for compounds inducing >10% luciferase activity at 20 μg/ml concentration (50 μM for a molecular weight of 400); no compound exhibiting such a level of activity was found in the library, except for ABA (Fig. 2A).This finding demonstrated that the screening system does not yield false positives and is suitable for chemical screening.We next searched for compounds that inhibited the cold induction of luciferase activity.Luciferase activity in DMSO-treated leaves exposed to low temperature was set to 100%, and CHX (70 μM) was used as a positive control for inhibition.Then, we screened for compounds (20 μg/ml; Supplemental Table S1) that caused at least a 90% inhibition of luciferase activity after low-temperature treatment (Fig. 2B).In the first screening round, 19 compounds met this criterion.We also included 18 additional compounds in the second screening.These 18 compounds did not meet the above criteria during the first screening; however, 1 of the tested leaves exhibited extremely low luciferase activity during the first screening.In total, we tested 37 compounds in the second screening.We measured the average of 4 independent leaves in the second screening (Supplemental Fig. S5), which allowed us to identify 4 compounds (20 μg/ml concentration) that inhibited luciferase activity by >99% during cold acclimation (Supplemental Table S2).
One of these compounds was CHX (Supplemental Table S2), an inhibitor of protein synthesis, which was used as the positive control for inhibition, further confirming the validity of this screening system.Among the remaining 3 compounds, we noticed that 2 compounds, menadione and plumbagin, were 1,4-naphthoquinones and quite similar in terms of structure (Fig. 2C).

Cold-regulated gene expression is strongly inhibited by 1,4-naphthoquinones
To investigate whether menadione and plumbagin indeed inhibit cold-regulated gene expression, we examined the cold induction of endogenous COR15A in the presence of these compounds.Endogenous COR15A expression was strongly induced during cold acclimation.However, when the plants were treated with menadione or plumbagin during cold acclimation, the induction of endogenous COR15A expression was inhibited (Fig. 3A).Similar observations were made for other cold-regulated genes, such as COLD-REGULATED 413 INNER MEMBRANE 1 (COR413IM1), INDUCIBLE2 (KIN2), RESPONSIVE TO DEHYDRATION 29A (RD29A), and CHALCONE SYNTHASE (CHS) (Kurkela and Borg-Franck 1992;Yamaguchi-Shinozaki and Shinozaki 1993;Okawa et al. 2008), albeit to varying degrees (Fig. 3A).
We also investigated the possible effects of these compounds on the freezing tolerance of Arabidopsis by testing electrolyte leakage from true leaves.We examined the freezing tolerance of cold-acclimated leaves in the presence and absence of the test compounds.As shown in Fig. 3B, the overall trend was that compound-treated plants were more susceptible to freezing stress.Likewise, we also investigated the freezing tolerance of whole plants.When Arabidopsis plants were cold acclimated in the presence of menadione or plumbagin, both menadione-and plumbagin-treated plants were more susceptible to freezing stress (Figs.3C and S6).Taken together, these observations are consistent with the hypothesis that menadione and plumbagin negatively regulate cold acclimation in Arabidopsis by affecting cold-regulated gene expression.
The effects of 1,4-naphthoquinones on cold-regulated gene expression were further evaluated using reverse transcription quantitative PCR (RT-qPCR) of endogenous genes.The expression of COR15A, COR413IM1, and CHS was induced by low-temperature treatment; however, this Natural compounds were obtained from the Natural Product Library (ENZO), and the list is shown in Supplemental Table S1.The numbers on the x-axis correspond to the identification number for each compound, as shown in Supplemental Table S1; 100 μM ABA and DMSO were used as positive and negative controls, respectively.Luciferase   induction was inhibited by 2CNQ and 5HNQ (Fig. 4C), consistent with the observations for menadione and plumbagin (Fig. 3A).In contrast, other quinones, such as DMBQ and 1,2NQ, did not efficiently inhibit the induction of cold-regulated genes at low temperatures (Fig. 4C).Consistent with this observation, the freezing tolerance of DMBQ-treated Arabidopsis was comparable to that of DMSO-treated control plants (Supplemental Fig. S6).This finding also confirmed that the impaired freezing tolerance of menadione-and plumbagin-treated plants was not caused by the general cytotoxicity of quinones.
Taken together, these data suggested that 1,4naphthoquinones specifically inhibit the cold induction of COR genes.Furthermore, this observation was not attributed to the cytotoxicity of 1,4-naphthoquinones.

Rapid CBF induction is inhibited by 1,4-naphthoquinones
Next, we investigated the possible mechanism whereby 1,4-naphthoquinones inhibited the induction of cold-regulated genes.CBF is a member of the APETALA2/ Ethylene Responsive Factor (AP2/ERF) transcription factor family and is a key regulator of cold signaling in plants (Stockinger et al. 1997;Jaglo-Ottosen et al. 1998).CBF1, CBF2, and CBF3 are induced transiently and rapidly in response to low temperatures, as well as regulate the expression of COR genes by binding to the C-repeat/ dehydration-responsive element (Gilmour et al. 1998).Therefore, we investigated the effects of 1,4-naphthoquinones on the rapid induction of CBF genes.When Arabidopsis plants were exposed to 4 °C for 4 h, CBF gene expression was strongly induced (Fig. 5).This effect was diminished by the simultaneous treatment with 1,4-naphthoquinones (Fig. 5, A and B).Other quinones, such as DMBQ and 1,2NQ, were ineffective at inhibiting CBF expression (Fig. 5, C and D).
We also investigated other transcription factors that might affect the expression of CBFs.It has been shown that the overexpression of ZINC FINGER OF ARABIDOPSIS THALIANA 12 (ZAT12), MYB DOMAIN PROTEIN 15 (MYB15), or ETHYLENE-INSENSITIVE 3 (EIN3) impairs the cold induction of CBF gene expression (Vogel et al. 2005;Agarwal et al. 2006;Shi et al. 2012).In this study, the expression of ZAT12 and MYB15 was strongly induced by 1,4-naphthoquinones under low temperature stress (Fig. 5).However, this was also observed for the other quinones (Fig. 5).The expression of EIN3 did not respond to either low-temperature or quinone treatment.Hence, 1,4-naphthoquinones appear to affect the expression of CBFs through a mechanism that is yet to be uncovered.
Taken together, these data indicated that 1,4-naphthoquinones inhibit cold signaling by affecting the induction of CBF genes.The data also suggested that the potential target of 1,4-naphthoquinones is the upstream component(s) involved in the perception and signaling of low temperatures.

Discussion
In the chemical screening of Arabidopsis, a major approach has been to germinate plants in microplates under sterile conditions and perform compound treatment shortly after germination (Armstrong et al. 2004;Kailasam et al. 2018;Jay et al. 2019;Uehara et al. 2019;Shirakawa et al. 2021).This approach has been used not only for reporter-based identification of chemical compounds but also for the phenotype-based identification (Jay et al. 2019).However, the life cycle of Arabidopsis continues thereafter.Thus, it is important to screen for compounds that affect environmental adaptation at later stages.Two major problems must be addressed.The first challenge is to obtain a compound in the cell beyond the barrier of the cuticular layer.The second issue is whether excised plant tissues exhibit the same environmental signal response as that of intact plants.Overcoming these challenges can greatly increase the value of chemical biology in plant science.Here, we developed a simple and high-throughput screening method to identify chemical compounds that affect cold acclimation, using excised leaves possessing a luciferase reporter construct.We demonstrated that this method is reliable and reproducible, as evidenced by the absence of false positives when screening for compounds upregulating COR15Apro::LUC expression in the absence of cold exposure (Fig. 2A).Luciferase activity was quantified directly without any image processing software (Supplemental Fig. S2C).Differences in leaf size and age did not affect either the cold induction of COR15Apro:: LUC or the effect of the inhibitor (Supplemental Figs.S3  and S4).There are 2 important aspects of this simple and highly reproducible screening method.First, plants were cultured in liquid medium before compound treatment (Fig. 1B).Second, cold-regulated gene expression in excised leaves was comparable to that in intact plants (Fig. 1E).This method is also versatile with respect to equipment.Luciferase activity can be also quantified with a CCD camera (Supplemental Fig. S4), as luciferase activity measured by CCD camera is consistent with that measured using a microplate reader (compare Supplemental Figs.S2C and S4).Overall, our method greatly facilitates the screening of chemical compounds that regulate abiotic stress responses.
Furthermore, our study provides insights into cold signaling in Arabidopsis.We identified chemical compounds that inhibit cold acclimation in Arabidopsis via chemical screening.The identification of such compounds offers a tool to further uncover the most upstream events of lowtemperature signaling.Photoreceptors such as phytochromes and phototropins serve as temperature sensors (Jung et al. 2016;Legris et al. 2016;Fujii et al. 2017).However, the temperature at which cold acclimation is Chemical screening method using single leaves PLANT PHYSIOLOGY 2023: 193; 234-245 | 241 strongly induced is much lower than the temperature range at which phytochromes are thought to function (Uemura et al. 1995).The freezing tolerance of Arabidopsis phot1phot2 mutants is unaffected after cold acclimation (Imai et al. 2021), suggesting that phototropins are less likely to play key roles in temperature sensing during cold acclimation.In rice (Oryza sativa), CHILLING-TOLERANCE DIVERGENCE 1 (COLD1) has been proposed to serve as a temperature sensor (Ma et al. 2015); however, the role of the Arabidopsis homolog GTG1/GTG2 in temperature sensing remains unclear (Pandey et al. 2009).
An intriguing hypothesis is that the intracellular target of 1,4-naphthoquinones is the upstream component of lowtemperature signaling, including low-temperature sensors.This hypothesis is supported by the fact that the rapid induction of CBF transcription factors was effectively inhibited by 1,4-naphthoquinones but not by other quinones (Fig. 5).In a recent study, it has been shown that a leucine-rich repeat receptor kinase, CANNOT RESPOND TO DMBQ 1 (CARD1), perceives DMBQ, a benzoquinone, to trigger defense-related gene expression and immune response to bacterial pathogens (Laohavisit et al. 2020).Another leucine-rich repeat receptor kinase, RECEPTOR-LIKE PROTEIN KINASE1 (RPK1), has been implicated in ABA perception at the plasma membrane (Osakabe et al. 2005;Konopka-Postupolska and Dobrowolska 2020;Shang et al. 2020).Our data also indicated that the rapid induction of COR15Apro::LUC by ABA was strongly inhibited by 2CNQ (Supplemental Fig. S7).Taken together, it appears that 1,4-naphthoquinones act on the upstream components at the plasma membrane, such as leucine-rich repeat receptor kinases that trigger lowtemperature and ABA signaling.As such, chemical screening using our method and further analysis using related compounds can greatly facilitate the understanding of the signaling pathways of interest.
In summary, we established a high-throughput screening method using single leaves of mature plants.This method allows us to use intact tissue excised from whole plants for chemical screening and may be applicable to other plant species that cannot germinate within microwell plates under sterile conditions.To date, fewer compound screenings have been performed in plants than in mammals, partly due to the lack of sophisticated experimental systems, as new medical drugs have not yet been developed for plants.
Our study offers a chemical screening scheme for identifying compounds that regulate plant growth and development.

Construction of plant transformation vectors and Arabidopsis (A. thaliana) transformation
A schematic of the COR15Apro::LUC construct is shown in Fig. 1A.The COR15A upstream region (980 bp from the start codon) and luciferase gene were amplified by PCR using KOD DNA polymerase (TOYOBO).The primers used to amplify each fragment are listed in Supplemental Table S3.Multiple fragments were subcloned into HindIII-XbaI sites of a modified pBI121 vector using an In-Fusion HD Cloning Kit (Takara) to create a COR15Apro::LUC construct fused with the NOS terminator.The construct was introduced into Arabidopsis (Col-0) via Agrobacterium-mediated transformation, using the floral dip method (Clough and Bent 1998).

Whole-plant submerged culture
For whole-plant submerged culture, ∼20 Arabidopsis seeds were directly sown in 50 mL of 0.5× MS medium containing 1% (w/v) sucrose (Tokumaru et al. 2017).To synchronize germination, flasks containing the medium and seeds were kept at 4 °C for 2 d after sowing.Plants were grown under continuous white light (60-80 μmol m −2 s −1 ) for 13-14 d at 22 °C in a growth chamber (LHP-220S or LHP-350S, NK Systems).Whole cultures or excised leaves were treated with each compound.Typical leaves used in chemical screening are shown in Supplemental Fig. S2.

Screening of compounds using transgenic COR15Apro:LUC plants
A natural product library (Supplemental Table S1) was purchased from ENZO life Sciences.Chemical screening was conducted using a 96-well microplate containing 200 μL of 0.5× MS liquid medium with 0.5 mM luciferin.A single true leaf excised from plants grown in submerged cultures was placed in each well and treated with the compound, as described below.The final concentration of each compound was 20 μg/ mL during the first screening (Fig. 2).Luciferase activity was measured using a Varioskan Flash microplate reader (Thermo Fisher Scientific).
For the first screening of compounds that induce the expression of COR15Apro::LUC (Fig. 2A), the leaves were treated with each compound for 4 h at 22 °C.As positive and negative controls, 4 leaves in each microplate were treated with ABA at a final concentration of 100 μM or DMSO, respectively.
For the first screening of compounds that inhibited the expression of COR15Apro::LUC at low temperatures (Fig. 2B), leaves were treated with compounds for 24 h at 4 °C.As positive and negative controls, 4 leaves from each microplate were treated with CHX at a final concentration of 70 μM or DMSO, respectively.During the first screening, the average luciferase activity of at least 2 independent leaves was calculated to evaluate the effect of each compound.
The second screening (Supplemental Fig. S5) and screening of quinone compounds (Fig. 4B) were similar, with the exception that 4 independent leaves were used for the assays.Selection criteria of compounds for the second screening were as follows: 19 compounds that caused at least a 90% inhibition of luciferase activity after low-temperature treatment and 18 compounds that 1 of the tested leaves exhibited extremely low luciferase activity.

RNA extraction and RT-qPCR analysis
For the experiments presented in Fig. 1, plants were grown in submerged cultures.The excised true leaves were then treated with low temperature and/or compounds in a microwell plate.The leaves were harvested and immediately frozen in liquid nitrogen.
For the experiments presented in Figs. 3 to 5, plants were grown in submerged cultures and then treated with low temperature and/or compounds in each flask for the indicated period.To cool the MS liquid medium immediately, the flasks were placed on ice-cold water for the first 30 min.After treatment, leaves were harvested and immediately frozen in liquid nitrogen.
In both cases, total RNA was isolated using RNAiso reagent (Takara) and cDNA was synthesized using the PrimeScript RT reagent kit (TaKaRa) with random hexamer and oligo(dT) primers.Then, RT-qPCR was performed on a Thermal Cycler Dice Real-Time System TP870 (TaKaRa) using TB Green Premix Ex Taq II (TaKaRa) or Brilliant III Ultra-Fast SYBR Green (Agilent) qPCR kits.Primers used for RT-qPCR are listed in Supplemental Table S4.The transcript level of each gene was normalized to that of ACTIN2.

Electrolyte leakage assay
The electrolyte leakage assay was performed as described previously (Minami et al. 2015).Plants grown in submerged cultures were treated with the compounds at 4 °C for 2 d in each flask.To cool the MS liquid medium immediately, the flasks were placed on ice-cold water for the first 30 min.Prior to the freezing assay, excess MS medium was removed from the plants by immersion in ice-cold water.Electrolyte leakage from the test leaves was measured using a conductivity meter as EL [Freezing temperature] .To obtain values for 100% electrolyte leakage, the samples were boiled at 95 °C for 20 min.The percentage of electrolyte leakage from leaves was determined by the ratio of EL [Freezing Temperature] to EL [100] (EL [Freezing temperature] /EL [100] × 100).

Whole-plant freezing tolerance assay
Wild-type plants grown in submerged culture for 11 d were cold acclimated at 4 °C for 2 d under continuous light in the presence or absence of chemical compounds.For nonacclimated control (Supplemental Fig. S1), plants were grown in submerged culture for 13 d.Then, plants were transferred to 0.5× MS medium containing 1% (w/v) sucrose and 0.8% (w/v) agar.MS agar plates were incubated at −2 °C using a low-temperature incubator Tokyo Rikakikai Co.,Ltd.,Tokyo,Japan).After 1 h of incubation, ice crystal formation was induced on plates using a spatula cooled with liquid nitrogen.The plates were incubated at −2 °C for another 1 h and were then cooled at a rate of −1 °C per 30 min to −10 °C.Once the temperature reached −10 °C, plates were incubated at the same temperature for another 1 h.The samples were then thawed overnight at 4 °C, returned to the growth chamber, and allowed to grow under normal conditions for ∼60 h.

Statistical analysis
At least 3 independent biological replicates were sampled for each analysis.Statistical significance was determined using Student's t-test formula in Microsoft Excel.P < 0.05 was considered significantly different.

Figure 1 .
Figure 1.Excised leaves of Arabidopsis grown in submerged cultures can respond to low temperature.A) Schematic of the COR15A promoter and luciferase fusion construct (COR15Apro::LUC) used in this study.B) Plants grown in the submerged culture (left).Excised leaves were placed in a 96-well microplate (right) and treated with low temperature and/or chemical compounds.C) Phenotype (upper) and bioluminescence (lower) of COR15Apro::LUC plants grown in submerged cultures after cold acclimation (CA).A nonacclimated plant (NA) was used as the control.D) Excised leaves of COR15Apro::LUC plants grown in submerged cultures were treated with low temperature (CA in left panel) or 100 μM ABA (middle panel).Plants were also treated with low temperature in the presence of DMSO or 70 μM CHX (right panel).Luciferase activity was measured using a Varioskan Flash microplate reader.The bars represent SE of the mean (n ≥ 3).E) Response of endogenous COR15A to low temperature in leaves excised from COR15Apro::LUC plants grown in submerged cultures.The bars represent SE of the mean (n = 3).

Figure 2 .
Figure 2. Screening of natural compounds that affect the expression of COR15Apro::LUC.A) Screening of natural compounds that induce the expression of COR15Apro::LUC without low-temperature treatment.Natural compounds were obtained from the Natural Product Library (ENZO), and the list is shown in Supplemental TableS1.The numbers on the x-axis correspond to the identification number for each compound, as shown in Supplemental TableS1; 100 μM ABA and DMSO were used as positive and negative controls, respectively.Luciferase (LUC) activity in ABA-treated plants was set to 100%.A red arrow indicates ABA in the library.B) Screening of natural compounds that inhibit the expression of COR15Apro::LUC during low-temperature treatment.Screening was performed as in panel A).The LUC activity in DMSO-treated plants was set to 100%; 70 μM CHX and DMSO were used as positive and negative controls, respectively.Red arrows indicate menadione (M) and plumbagin (P) in the library.C) Chemical structures of menadione and plumbagin.
Figure 2. Screening of natural compounds that affect the expression of COR15Apro::LUC.A) Screening of natural compounds that induce the expression of COR15Apro::LUC without low-temperature treatment.Natural compounds were obtained from the Natural Product Library (ENZO), and the list is shown in Supplemental TableS1.The numbers on the x-axis correspond to the identification number for each compound, as shown in Supplemental TableS1; 100 μM ABA and DMSO were used as positive and negative controls, respectively.Luciferase (LUC) activity in ABA-treated plants was set to 100%.A red arrow indicates ABA in the library.B) Screening of natural compounds that inhibit the expression of COR15Apro::LUC during low-temperature treatment.Screening was performed as in panel A).The LUC activity in DMSO-treated plants was set to 100%; 70 μM CHX and DMSO were used as positive and negative controls, respectively.Red arrows indicate menadione (M) and plumbagin (P) in the library.C) Chemical structures of menadione and plumbagin.

Figure 3 .
Figure 3. Menadione and plumbagin affect cold acclimation in Arabidopsis.A) Effects of 50 μM menadione and 30 μM plumbagin on cold-regulated gene expression in 2-d cold-acclimated Arabidopsis plants grown in submerged culture.The PYRUVATE DEHYDROGENASE E1α SUBUNIT (PDHE1α) gene was used as a control.Total RNA was extracted from true leaves, and mRNA levels were analyzed by RT-qPCR.The bars represent SE of the mean (n = 3).The expression levels in the cold-acclimated (CA) plants in each experimental set were set to 1. Significant differences between CA and compound-treated plants are indicated by asterisks (*P < 0.05 as determined by Student's t-test).B) Effects of 50 μM menadione and 30 μM plumbagin on electrolyte leakage of 2-d cold-acclimated true leaves.True leaves were excised from compound-treated plants grown in submerged cultures, placed in a test tube, and then cooled to the indicated temperatures using a programmable freezer as described in the Materials and methods.Values are the means of at least 3 independent leaves, with bars representing SE.Significant differences from DMSO-treated plants are indicated by asterisks (*P < 0.05 as determined by Student's t-test).C) Phenotype of Arabidopsis subjected to freezing stress.Plants were treated with DMSO, 50 μM menadione or 30 μM plumbagin in submerged cultures during cold acclimation and then subjected to freezing stress (−10 °C) on MS agar plates.Control plants were returned to growth chamber without freezing stress.Representative plants were selected from whole plate images shown in Supplemental Fig. S6.