Validating Rat Model of Polycystic Ovary Syndrome Induced Non-Alcoholic Fatty Liver Disease: Role of Hyperandrogenism and Insulin Resistance

Background

Polycystic ovary syndrome (PCOS) is a highly prevalent endocrinemetabolic disorder. PCOS is frequently associated with abdominal adiposity, insulin resistance, and cardiovascular risk factors. Increased prevalence of NAFLD has been reported in patients with PCOS.

Aim

to validate a model of PCO induced NAFLD and determine the role of androgen and insulin resistance in the model pathogenesis.

Material and Method

Testosterone Enanthate (TE) was used to induce PCOS in female wistar rats by receiving TE subcutaneously at a dose of 7.5 mg dissolved in sesame oil by S.C. injection daily from embryonic day 16 (E16) to E19, female offsprings are divided into; control group, model group.

Results

PCO produced significant increase in body weight, liver weight, ALT/AST ratio, testosterone (T), Luteinizing hormone (LH), fasting glucose, serum insulin, HOMA-IR and serum c-peptide with dyslipidemia and decrease in serum estradiol (E₂) level. The picture of PCOS and NAFLD was clearly seen biochemically and histologically.

Conclusion

our study validated the model of PCO induced NAFLD as manifested by the biochemical and histological analysis. .

Polycystic ovary syndrome, Non-alcoholic fatty liver disease, testosterone enanthate, insulin resistance, androgen