organ-on-a-chip

...-acting elements (5'-AAAAAG-3') in the promoters of target 507 genes [12]. We analyzed the MdCKX7 promoter and obtained four potential 508 binding sites of DOF transcription factors, namely P1, P2, P3, and P4. 509 Chromatin immunoprecipitation PCR (ChIP-PCR) assay was performed to 510 identify the binding...

... starting DNA input was 150 ng, per clinical protocol. Genome-wide DNA methylation profiling was performed using the Infinium Methylation EPIC (EPIC) BeadChip or Infinium Human Methylation Bead Chip arrays (Illumina), and FFPE restore kit, according to the manufacturer s instructions as described...

..., governments, and other organizations often feature an “executive summary” similar to our treatment. After reading the text, treatment group participants completed a question asking them to select all items that the report considers “a threat to democracy in the United States.” The question has six answer...

... 1907, reform efforts intensified: during the next decade, the United States boasted more reform organizations devoted to the abolition of capital punishment than ever since the 1840s, prominent Americans spoke out in favor of abolition, and by 1917, capital punishment had been abolished in twelve...

... structure27 based capacity of those tsRNAs to organize stress granules may prevent cellular death upon 5 ACCEPTED MANUSCRIPT 1 exposure to acute cellular stressors43. Another report highlighted the neuroprotective role of the 2 same angiogenin-cleaved tsRNA-Ala and supported its involvement in stress granule...

... of degradation, such as Western lifestyles or even the phase-out of fossil fuels, rendered non-negotiable? Why is there hardly any political attention paid to truly systemic shifts, to alternative forms 2 Captured Futures of economic or social organization? Why do we see hardly any images of what sustainable...

... with high organ parasite burden and anti-inflammatory cytokines along with increased 11β-hydroxysteroid dehydrogenase (11βHSD1) expression. DHEA treatment in J774 and THP-1-derived macrophages restrains L. donovani-induced cortisol levels by inhibiting 11βHSD1 expression that increases pro...

... cells were cultured in microfluidic chips to form functional tubes. After inducing fibrotic changes by stimulation with an idiopathic pulmonary fibrosis-relevant cytokine cocktail, functional and molecular readouts were performed to analyse the effect of treatment with nerandomilast. Bleo-induced lung...

... as an institution that creates male heroes and as a punishment for the worst of the worst—in the case of the 1950s: organized crime. Ambiguous films and a cluster that contains both clearly abolitionist and retentionist films shows that Hollywood was not as politically progressive as is often thought. In a last...

.... Defamiliarization is a strategy that chips away at the dominance and self-evidence of prevailing power structures. It may not provide concrete ideas or discourses about how the world should be organized (as epochal storytelling or backgrounding CO₂ might), but it nonetheless re-values...

... with immune cell types and states, researchers can better understand the molecular mechanisms underlying neuroinflammation and neurodegeneration. [108] Accepted Manuscript Epigenetic studies:Epigenetics can be studied using techniques like ATAC-seq, which maps open chromatin regions, and ChIP-seq, which...

.... This resembles, though is not identical to, how arbitration organizations in the US successfully sought to embed private due process norms into their domestic arbitrations (there the method was by refusing, as private actors, to administer arbitrations if the arbitral procedures did not comply with these norms...

... University Press, Standard Journals Publication Model ( https://academic.oup.com/pages/standard-publication-reuse-rights ) Abstract Background/Aims Glucocorticoids play a pivotal role in the management of active SLE; however, their use is associated with the risk of irreversible organ damage. The lack...

... sword in sepsis since they contribute to both hyperinflammatory and immunosuppressive phases. Our current knowledge regarding neutrophil heterogeneity in sepsis is summarized in Fig. 2 . A recent study using an organ-on-chip assay identified three distinct subsets (hyperimmune, hypoimmune, and hybrid...

... V bias voltage). This new paradigm of mechanistic study and reaction optimization shows great potential applications in scalable synthesis by integrated single-molecule electronic devices on chip. Real-time single-molecule electrical monitoring at the nanosecond scale reveals hidden reaction steps...

... with a C57BL/6J mouse to generate Dyrk1b-flox mice. These mice were crossed with Alb-Cre transgenic mice to produce HepKO mice, and Dyrk1b-flox (Flox) mice were used as controls. All db/db and ob/ob mice and matched lean littermate controls (db/WT, ob/WT) were obtained from Shanghai Model Organisms Center...

... of omics data have been generated in diverse biological research fields, which provides valuable opportunities for investigating the underlying mechanisms of tissue development or disease progression. For example, the encyclopedia of DNA elements (ENCODE) project [ 1 ] provided ChIP-seq and DNase-seq data...