Protective Effect of Quercetin on Aroclor 1254–Induced Oxidative Damage in Cultured Chicken Spermatogonial Cells

Abstract

Quercetin, a dietary-derived falvonol-type flavonoid, is ubiquitous in fruits and vegetables and plays important roles in human health by virtue of its antioxidant function. The present study was performed to investigate effects of quercetin on oxidative damage that was induced by an environmental endocrine disrupter, Aroclor 1254 (A1254), in cultured spermatogonial cells of embryonic chickens. Spermatogonial cells were dispersed from 18-day-old embryo and exposed to A1254 alone or in combination with quercetin. The oxidative damage was estimated by measuring contents of thiobarbituric acid–reactive substances (TBARS, an indicator of lipid peroxidation), activity of superoxide dismutase (SOD, a scavenger of superoxide), and activity of glutathione (GSH, an intracellular antioxidant). Results showed that quercetin had no deleterious effect on spermatogonial cells at 0.01∼1 μg/ml. Exposure to A1254 (10 μg/ml) induced an increase of spermatogonial cell number, and membrane integrity was damaged by elevation of lactate dehydrogenase (LDH) leakage. Exposure to A1254 also induced an elevation in TBARS but a decrease in SOD activity and GSH content. However, compared with A1254 treatment alone, simultaneous supplementation with quercetin decreased LDH leakage to maintain the cell integrity, decreased the levels of TBARS to quench the free radicals, increased SOD activity and GSH content to restore the endogenous antioxidant defense system. Thus, quercetin displayed protective effects on spermatogonial cells from A1254-induced oxidative damage through increasing intracellular antioxidant levels and decreasing lipid peroxidation. Consequently, the antioxidant, such as quercetin, from food or feed consumed by human and animals may attenuate the negative effects of environmental endocrine disrupters.

quercetin, polychlorinated biphenyls, oxidative damage, chicken, spermatogonial cells