Kinetic Parameters of CL11, CL11-M, and CL11-E Binding to 58rRNA Determined by SPR.
| Protein . | kon (1/M×s) . | koff (1/s) . | KD (M) (Koff/Kon) . |
|---|---|---|---|
| CL11 | 1.2 ± 0.4×104 | 3.0 ± 0.2×10−4 | 2.5 ± 0.6×10−8 |
| CL11-M | 1.9 ± 0.9×104 | 4.5 ± 0.9×10−4 | 2.4 ± 0.9×10−8 |
| CL11-E | 1.6 ± 0.8×103 | 3.6 ± 0.8×10−6 | 2.2 ± 0.3×10−9 |
| Protein . | kon (1/M×s) . | koff (1/s) . | KD (M) (Koff/Kon) . |
|---|---|---|---|
| CL11 | 1.2 ± 0.4×104 | 3.0 ± 0.2×10−4 | 2.5 ± 0.6×10−8 |
| CL11-M | 1.9 ± 0.9×104 | 4.5 ± 0.9×10−4 | 2.4 ± 0.9×10−8 |
| CL11-E | 1.6 ± 0.8×103 | 3.6 ± 0.8×10−6 | 2.2 ± 0.3×10−9 |
The SD (±) was calculated from two different sets of experiments each with three different protein concentrations.
Kinetic Parameters of CL11, CL11-M, and CL11-E Binding to 58rRNA Determined by SPR.
| Protein . | kon (1/M×s) . | koff (1/s) . | KD (M) (Koff/Kon) . |
|---|---|---|---|
| CL11 | 1.2 ± 0.4×104 | 3.0 ± 0.2×10−4 | 2.5 ± 0.6×10−8 |
| CL11-M | 1.9 ± 0.9×104 | 4.5 ± 0.9×10−4 | 2.4 ± 0.9×10−8 |
| CL11-E | 1.6 ± 0.8×103 | 3.6 ± 0.8×10−6 | 2.2 ± 0.3×10−9 |
| Protein . | kon (1/M×s) . | koff (1/s) . | KD (M) (Koff/Kon) . |
|---|---|---|---|
| CL11 | 1.2 ± 0.4×104 | 3.0 ± 0.2×10−4 | 2.5 ± 0.6×10−8 |
| CL11-M | 1.9 ± 0.9×104 | 4.5 ± 0.9×10−4 | 2.4 ± 0.9×10−8 |
| CL11-E | 1.6 ± 0.8×103 | 3.6 ± 0.8×10−6 | 2.2 ± 0.3×10−9 |
The SD (±) was calculated from two different sets of experiments each with three different protein concentrations.
This PDF is available to Subscribers Only
View Article Abstract & Purchase OptionsFor full access to this pdf, sign in to an existing account, or purchase an annual subscription.
