Redundancy analysis showing percent variation in the wastewater resistome composition explained by biotic and abiotic variables
| . | Gene composition . | Transcript composition . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Influent . | Effluent . | Influent . | Effluent . | ||||||||
| . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . |
| Biotic variables | ||||||||||||
| intI1/16S | 34.5 | 28.7 | 27.9 | 27.8 | 22.5 | 32.1 | 24.9 | 18.8 | 18.8 | 33.2 | 16.7 | 18.9 |
| Resolvase/16S | 35.7 | 31.1 | 30.1 | 26.3 | 20.4 | 32.5 | 28.5 | 20.8 | 21.2 | 39.1 | 14.8 | 18.9 |
| CTP/16S | 34.5 | 29.4 | 28.9 | 28.6 | 24.8 | 35.7 | 25.6 | 20.1 | 19.2 | 42.1 | 17.6 | 21.1 |
| Shannon’s H | 29.4 | 25.7 | 33.6 | 40.3 | 17.1 | 20.6 | ||||||
| Simpson’s E | 14.2 | 14.1 | 16.1 | 30.8 | 10.4 | |||||||
| Abiotic variables | ||||||||||||
| VSS (mg/L) | 13.3 | 9.8 | 14.1 | 7.9 | 13.7 | 11.8 | 16.3 | 16.9 | 10.4 | |||
| Nitrate nitrogen (mg/L) | 10.8 | |||||||||||
| Total nitrogen (mg/L) | 12.3 | |||||||||||
| pH | 24.7 | 19.4 | ||||||||||
| Ciprofloxacin (ng/L) | 14.5 | 15.9 | ||||||||||
| Triclosan (ng/L) | 10.3 | |||||||||||
| . | Gene composition . | Transcript composition . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Influent . | Effluent . | Influent . | Effluent . | ||||||||
| . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . |
| Biotic variables | ||||||||||||
| intI1/16S | 34.5 | 28.7 | 27.9 | 27.8 | 22.5 | 32.1 | 24.9 | 18.8 | 18.8 | 33.2 | 16.7 | 18.9 |
| Resolvase/16S | 35.7 | 31.1 | 30.1 | 26.3 | 20.4 | 32.5 | 28.5 | 20.8 | 21.2 | 39.1 | 14.8 | 18.9 |
| CTP/16S | 34.5 | 29.4 | 28.9 | 28.6 | 24.8 | 35.7 | 25.6 | 20.1 | 19.2 | 42.1 | 17.6 | 21.1 |
| Shannon’s H | 29.4 | 25.7 | 33.6 | 40.3 | 17.1 | 20.6 | ||||||
| Simpson’s E | 14.2 | 14.1 | 16.1 | 30.8 | 10.4 | |||||||
| Abiotic variables | ||||||||||||
| VSS (mg/L) | 13.3 | 9.8 | 14.1 | 7.9 | 13.7 | 11.8 | 16.3 | 16.9 | 10.4 | |||
| Nitrate nitrogen (mg/L) | 10.8 | |||||||||||
| Total nitrogen (mg/L) | 12.3 | |||||||||||
| pH | 24.7 | 19.4 | ||||||||||
| Ciprofloxacin (ng/L) | 14.5 | 15.9 | ||||||||||
| Triclosan (ng/L) | 10.3 | |||||||||||
Only variables and values with significant constraints in the RDA tests (P < 0.05, 1000 permutation) are shown, and a full list of the tested variables are available in Dataset S5
intI1 class 1 integron-integrase gene, 16S, 16S ribosomal RNA gene, CTP conjugal transfer protein-coding gene, VSS volatile suspended solids
Redundancy analysis showing percent variation in the wastewater resistome composition explained by biotic and abiotic variables
| . | Gene composition . | Transcript composition . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Influent . | Effluent . | Influent . | Effluent . | ||||||||
| . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . |
| Biotic variables | ||||||||||||
| intI1/16S | 34.5 | 28.7 | 27.9 | 27.8 | 22.5 | 32.1 | 24.9 | 18.8 | 18.8 | 33.2 | 16.7 | 18.9 |
| Resolvase/16S | 35.7 | 31.1 | 30.1 | 26.3 | 20.4 | 32.5 | 28.5 | 20.8 | 21.2 | 39.1 | 14.8 | 18.9 |
| CTP/16S | 34.5 | 29.4 | 28.9 | 28.6 | 24.8 | 35.7 | 25.6 | 20.1 | 19.2 | 42.1 | 17.6 | 21.1 |
| Shannon’s H | 29.4 | 25.7 | 33.6 | 40.3 | 17.1 | 20.6 | ||||||
| Simpson’s E | 14.2 | 14.1 | 16.1 | 30.8 | 10.4 | |||||||
| Abiotic variables | ||||||||||||
| VSS (mg/L) | 13.3 | 9.8 | 14.1 | 7.9 | 13.7 | 11.8 | 16.3 | 16.9 | 10.4 | |||
| Nitrate nitrogen (mg/L) | 10.8 | |||||||||||
| Total nitrogen (mg/L) | 12.3 | |||||||||||
| pH | 24.7 | 19.4 | ||||||||||
| Ciprofloxacin (ng/L) | 14.5 | 15.9 | ||||||||||
| Triclosan (ng/L) | 10.3 | |||||||||||
| . | Gene composition . | Transcript composition . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Influent . | Effluent . | Influent . | Effluent . | ||||||||
| . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . | ARG . | BRG . | MRG . |
| Biotic variables | ||||||||||||
| intI1/16S | 34.5 | 28.7 | 27.9 | 27.8 | 22.5 | 32.1 | 24.9 | 18.8 | 18.8 | 33.2 | 16.7 | 18.9 |
| Resolvase/16S | 35.7 | 31.1 | 30.1 | 26.3 | 20.4 | 32.5 | 28.5 | 20.8 | 21.2 | 39.1 | 14.8 | 18.9 |
| CTP/16S | 34.5 | 29.4 | 28.9 | 28.6 | 24.8 | 35.7 | 25.6 | 20.1 | 19.2 | 42.1 | 17.6 | 21.1 |
| Shannon’s H | 29.4 | 25.7 | 33.6 | 40.3 | 17.1 | 20.6 | ||||||
| Simpson’s E | 14.2 | 14.1 | 16.1 | 30.8 | 10.4 | |||||||
| Abiotic variables | ||||||||||||
| VSS (mg/L) | 13.3 | 9.8 | 14.1 | 7.9 | 13.7 | 11.8 | 16.3 | 16.9 | 10.4 | |||
| Nitrate nitrogen (mg/L) | 10.8 | |||||||||||
| Total nitrogen (mg/L) | 12.3 | |||||||||||
| pH | 24.7 | 19.4 | ||||||||||
| Ciprofloxacin (ng/L) | 14.5 | 15.9 | ||||||||||
| Triclosan (ng/L) | 10.3 | |||||||||||
Only variables and values with significant constraints in the RDA tests (P < 0.05, 1000 permutation) are shown, and a full list of the tested variables are available in Dataset S5
intI1 class 1 integron-integrase gene, 16S, 16S ribosomal RNA gene, CTP conjugal transfer protein-coding gene, VSS volatile suspended solids
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