Table 4.

Differential expression levels (fold change) of genes encoding proteins involved in suberin synthesisSuberin and wax biosynthesis.

Q. suber accession no.aFold changebBest TAIR BLASTXq-valueAnnotation
All_0001341.267At1g01600
At1g63710
At2g45970
At4g00360
2,90e–5Cytochrome P450, family 86 (CYP86A4S)
All_002989
All_004601
NDEAt3g10570Cytochrome P450, family 77 (CYP77A6)
All_000353
All_000345
All_000340
All_000356
NDE
1.283
1.504
NDE
At5g410400.105
2.74e–7
Omega-hydroxypalmitate O-feruloyl transferase
All_013100
All_013141
NDE
NDE
At1g70680Caleosin-related family protein
All_000132NDEPOPTR_588365Cytochrome P450, family 94 (CYP94A1)
All_000075NDEAt5g23190Cytochrome P450, family 86 (CYP86B1)
All_000025
All_000415
All_003882
All_005135
NDEAt4g16760
At2g35690
Long-chain-fatty-acyl-CoA reductase
--------At1g02205Aldehyde decarbonylase (CER1)
All_000205
All_000301
2.78
NDE
At3g445400.179Fatty acyl-CoA reductase
-----------At5g37300Diacylglycerol O-acyltransferase
Biosynthesis of unsaturated fatty acids
All_001650NDEAt1g01710
At4g00520
Palmitoyl-CoA hydrolase
Fatty acid elongation
All_000492
All_005669
All_003985
NDE
NDE
0.63
At2g33150
At5g47720
At5g48230
0.312Acetil-CoA acyltransferase
All_006584
All_008459
NDE
2.5
At3g152900.0033-hydroxyacyl-CoA dehydrogenase
All_002945
All_004827
All_002608
NDE
NDE
4
At1g605500.0001Enoyl-CoA hydratase
All_0031104.2At3g457700.332Trans-2-enoyl-CoA reductase
All_000415
All_006890
NDE
NDE
At3g51840
At3g06810
2-Enoyl-CoA reductase
All_000165
All_000113
All_000221
2.125
1.47
2.62
At1g01120
At1g04220
5.31e–9
7.3e–9
0.0002
3-ketocyl-CoA synthase (in endoplasmic reticulum)
All_0022431.33At1g677300.019Very-long-chain 3-oxoacyl-CoA
All_004052NDEAt5g10480Very-long-chain (BR)-3-hydroxyacyl dehydratase
All_0019134.8At3g553602.87e–13Very-long-chain enoyl-CoA reductase
Q. suber accession no.aFold changebBest TAIR BLASTXq-valueAnnotation
All_0001341.267At1g01600
At1g63710
At2g45970
At4g00360
2,90e–5Cytochrome P450, family 86 (CYP86A4S)
All_002989
All_004601
NDEAt3g10570Cytochrome P450, family 77 (CYP77A6)
All_000353
All_000345
All_000340
All_000356
NDE
1.283
1.504
NDE
At5g410400.105
2.74e–7
Omega-hydroxypalmitate O-feruloyl transferase
All_013100
All_013141
NDE
NDE
At1g70680Caleosin-related family protein
All_000132NDEPOPTR_588365Cytochrome P450, family 94 (CYP94A1)
All_000075NDEAt5g23190Cytochrome P450, family 86 (CYP86B1)
All_000025
All_000415
All_003882
All_005135
NDEAt4g16760
At2g35690
Long-chain-fatty-acyl-CoA reductase
--------At1g02205Aldehyde decarbonylase (CER1)
All_000205
All_000301
2.78
NDE
At3g445400.179Fatty acyl-CoA reductase
-----------At5g37300Diacylglycerol O-acyltransferase
Biosynthesis of unsaturated fatty acids
All_001650NDEAt1g01710
At4g00520
Palmitoyl-CoA hydrolase
Fatty acid elongation
All_000492
All_005669
All_003985
NDE
NDE
0.63
At2g33150
At5g47720
At5g48230
0.312Acetil-CoA acyltransferase
All_006584
All_008459
NDE
2.5
At3g152900.0033-hydroxyacyl-CoA dehydrogenase
All_002945
All_004827
All_002608
NDE
NDE
4
At1g605500.0001Enoyl-CoA hydratase
All_0031104.2At3g457700.332Trans-2-enoyl-CoA reductase
All_000415
All_006890
NDE
NDE
At3g51840
At3g06810
2-Enoyl-CoA reductase
All_000165
All_000113
All_000221
2.125
1.47
2.62
At1g01120
At1g04220
5.31e–9
7.3e–9
0.0002
3-ketocyl-CoA synthase (in endoplasmic reticulum)
All_0022431.33At1g677300.019Very-long-chain 3-oxoacyl-CoA
All_004052NDEAt5g10480Very-long-chain (BR)-3-hydroxyacyl dehydratase
All_0019134.8At3g553602.87e–13Very-long-chain enoyl-CoA reductase

Values below zero mean that the genes are more highly expressed in BQC. NDE, not differentially expressed.

aQ. suber accession number refers to the accession number assigned after pyrosequencing.

b Fold change is expressed as the ratio of gene expression of GQC to BQC.

Table 4.

Differential expression levels (fold change) of genes encoding proteins involved in suberin synthesisSuberin and wax biosynthesis.

Q. suber accession no.aFold changebBest TAIR BLASTXq-valueAnnotation
All_0001341.267At1g01600
At1g63710
At2g45970
At4g00360
2,90e–5Cytochrome P450, family 86 (CYP86A4S)
All_002989
All_004601
NDEAt3g10570Cytochrome P450, family 77 (CYP77A6)
All_000353
All_000345
All_000340
All_000356
NDE
1.283
1.504
NDE
At5g410400.105
2.74e–7
Omega-hydroxypalmitate O-feruloyl transferase
All_013100
All_013141
NDE
NDE
At1g70680Caleosin-related family protein
All_000132NDEPOPTR_588365Cytochrome P450, family 94 (CYP94A1)
All_000075NDEAt5g23190Cytochrome P450, family 86 (CYP86B1)
All_000025
All_000415
All_003882
All_005135
NDEAt4g16760
At2g35690
Long-chain-fatty-acyl-CoA reductase
--------At1g02205Aldehyde decarbonylase (CER1)
All_000205
All_000301
2.78
NDE
At3g445400.179Fatty acyl-CoA reductase
-----------At5g37300Diacylglycerol O-acyltransferase
Biosynthesis of unsaturated fatty acids
All_001650NDEAt1g01710
At4g00520
Palmitoyl-CoA hydrolase
Fatty acid elongation
All_000492
All_005669
All_003985
NDE
NDE
0.63
At2g33150
At5g47720
At5g48230
0.312Acetil-CoA acyltransferase
All_006584
All_008459
NDE
2.5
At3g152900.0033-hydroxyacyl-CoA dehydrogenase
All_002945
All_004827
All_002608
NDE
NDE
4
At1g605500.0001Enoyl-CoA hydratase
All_0031104.2At3g457700.332Trans-2-enoyl-CoA reductase
All_000415
All_006890
NDE
NDE
At3g51840
At3g06810
2-Enoyl-CoA reductase
All_000165
All_000113
All_000221
2.125
1.47
2.62
At1g01120
At1g04220
5.31e–9
7.3e–9
0.0002
3-ketocyl-CoA synthase (in endoplasmic reticulum)
All_0022431.33At1g677300.019Very-long-chain 3-oxoacyl-CoA
All_004052NDEAt5g10480Very-long-chain (BR)-3-hydroxyacyl dehydratase
All_0019134.8At3g553602.87e–13Very-long-chain enoyl-CoA reductase
Q. suber accession no.aFold changebBest TAIR BLASTXq-valueAnnotation
All_0001341.267At1g01600
At1g63710
At2g45970
At4g00360
2,90e–5Cytochrome P450, family 86 (CYP86A4S)
All_002989
All_004601
NDEAt3g10570Cytochrome P450, family 77 (CYP77A6)
All_000353
All_000345
All_000340
All_000356
NDE
1.283
1.504
NDE
At5g410400.105
2.74e–7
Omega-hydroxypalmitate O-feruloyl transferase
All_013100
All_013141
NDE
NDE
At1g70680Caleosin-related family protein
All_000132NDEPOPTR_588365Cytochrome P450, family 94 (CYP94A1)
All_000075NDEAt5g23190Cytochrome P450, family 86 (CYP86B1)
All_000025
All_000415
All_003882
All_005135
NDEAt4g16760
At2g35690
Long-chain-fatty-acyl-CoA reductase
--------At1g02205Aldehyde decarbonylase (CER1)
All_000205
All_000301
2.78
NDE
At3g445400.179Fatty acyl-CoA reductase
-----------At5g37300Diacylglycerol O-acyltransferase
Biosynthesis of unsaturated fatty acids
All_001650NDEAt1g01710
At4g00520
Palmitoyl-CoA hydrolase
Fatty acid elongation
All_000492
All_005669
All_003985
NDE
NDE
0.63
At2g33150
At5g47720
At5g48230
0.312Acetil-CoA acyltransferase
All_006584
All_008459
NDE
2.5
At3g152900.0033-hydroxyacyl-CoA dehydrogenase
All_002945
All_004827
All_002608
NDE
NDE
4
At1g605500.0001Enoyl-CoA hydratase
All_0031104.2At3g457700.332Trans-2-enoyl-CoA reductase
All_000415
All_006890
NDE
NDE
At3g51840
At3g06810
2-Enoyl-CoA reductase
All_000165
All_000113
All_000221
2.125
1.47
2.62
At1g01120
At1g04220
5.31e–9
7.3e–9
0.0002
3-ketocyl-CoA synthase (in endoplasmic reticulum)
All_0022431.33At1g677300.019Very-long-chain 3-oxoacyl-CoA
All_004052NDEAt5g10480Very-long-chain (BR)-3-hydroxyacyl dehydratase
All_0019134.8At3g553602.87e–13Very-long-chain enoyl-CoA reductase

Values below zero mean that the genes are more highly expressed in BQC. NDE, not differentially expressed.

aQ. suber accession number refers to the accession number assigned after pyrosequencing.

b Fold change is expressed as the ratio of gene expression of GQC to BQC.

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