Fig. 3
Cannabidiol (CBD) administration exerts an anxiolytic effect in a chronic unpredictable stress (CUS) paradigm. (a) Wild-type (WT) and glial fibrillary acidic protein thymidine kinase (GFAP-TK) transgenic mice were subjected to CUS or left undisturbed and time latency in the novelty suppression feeding test was determined after chronic administration of vehicle (Veh) or 30 mg/kg CBD (n = 14, 12, 6, 7, 5, 4, 3 and 4 animals per group, respectively). (b, c) The anxiolytic effect of CBD administration in mice subjected to CUS was also determined in the elevated plus-maze test. White bars represent the percentage of entries into the open arms; black bars represent the percentage of the time spent in the open arms (n = 4, 3, 4 and 4 animals per group, respectively). Entries in the enclosed arms were also quantified (c). * p < 0.05, ** p < 0.01 vs. the respective Veh-treated mice; # p < 0.05, ## p < 0.01 vs. the respective WT mice; ‡ p < 0.01 vs. the respective non-stressed mice (two-way analysis of variance followed by Duncan's post hoc test).